FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R1_D11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R1_D11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences840243
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGT450.00966461248.3003774
GGTCGTT1801.8189894E-1244.2648057
AGCGAAA2550.042.6662371
CGAAAGC2800.041.4027863
GTAGAAA5950.039.009131
GCGAAAG2950.036.8590162
AGGTCGT2103.8198777E-1034.4941066
CAATTCG2200.032.9242369
TATACGT1204.0737464E-430.1877334
CTTACAC3950.029.3400789
AGTAGAA6500.029.0130391
CTTAGAC6500.028.9819493
GACAGTA2752.1464075E-1028.9733287
CATACTC2553.0413503E-928.413683
TTAGACA5800.027.4812474
CAGCTCG3503.6379788E-1226.9038019
TTATAGG2705.5333658E-926.8639261
TAGACAG6800.026.6314815
ACTATAT4950.026.3472273
TTGTCGG1100.00971419326.3393889
Produced by FastQC (version 0.11.5)