FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R1_D10-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R1_D10-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences597466
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACAGAATGTGGCTTAAACATGGGATCATAGGTCTAAGCGGGCA16620.27817482501096297No Hit
GTCTAATATCTGAAGAATGACAGATTCACGCACATATACACAGAATGTGG10560.17674645921274182No Hit
GTCCTGAATGCCTCCTCCCATGGCTTGGTCCTCCACAGCTTGGAGCCTGC10120.1693820234122109No Hit
GTACAGGCTGGGGGCGAAGGCTCTGCTTTCACACCCGGGGGCGTTCCTCA9660.16168284052983767No Hit
GTACAAGCCTTACCCTGATGACGTTGGCCCTAGAGGAATCTGAGCTGCAT9310.1558247665975972No Hit
CCTTAGGGAAGGGATTGGCCACCGGCATCCGGGCATCCCGCAGCTCTTCG8950.14979931912443553No Hit
GTCCTGGGGCATCAGCATCCGGCTGTTCAGAAACTCTTCACGGGGAGGCT8900.14896245141982975No Hit
CCCTAAGAGGGGCTGAGTAGAGTCACAGCGGAGGTAATGCCCTGGACCTG7220.12084369654507536No Hit
AAATAGAAGTGAGAAAAGAAAAAGGAAGTAAAATACAAATATCTGGGCCT7060.11816571989033686No Hit
CCCTGGGCCCTGGCTTCTTCTCTTCTTCTTCTAGCACCGTGATCTTGGTG6690.11197289887625406No Hit
TATACACAGAATGTGGCTTAAACATGGGATCATAGGTCTAAGCGGGCACA6550.10962966930335785No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2750.092.328371
GCGAAAG2800.090.611292
CGAAAGC3000.084.528063
GGCGATC753.7800055E-757.9523937
TATAGTA400.006053318754.3667762
GCGATCT805.903057E-754.3303688
TAATGAT553.2296884E-452.6884044
GGGCGAT858.969364E-751.134466
TCATAGC700.001059701741.4014973
AACTATA750.001488903538.6349266
GATTATA1157.108405E-637.8488921
CAAGCCG1157.120683E-637.83938145
AGGGCGA1157.178216E-637.795045
ATGCGGA800.002044182536.2202459
CGATCTC1406.646278E-736.2202459
GATTACC900.00361637632.241651
GTAGGTA3150.032.1957745
TAGGGAA8550.031.351144
GCGTGGA1902.0769585E-730.5216962
GATGCGG950.004744807730.5012598
Produced by FastQC (version 0.11.5)