FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R1_D09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R1_D09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121
Sequences flagged as poor quality0
Sequence length151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTGTCCAAAATG21.6528925619834711No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA21.6528925619834711No Hit
TACAAGCATTGCAACAACTACTTCATTGCATGTTTGAGGAAGGAGTTGAA10.8264462809917356No Hit
ATGGTGGAGTGGAGAGCCAGAGGCTGCTGCCTGTGGTGAAGTCTCATCAG10.8264462809917356No Hit
ACTTCCCTCTCGCTTAGAATAACACATCTTAATAAATTTCATGTCCTTTT10.8264462809917356No Hit
GTTGGGGGGGGGGCTGGGGGGGGCGGTGGGCGGGGGGGGCGGGGGGGGCC10.8264462809917356No Hit
GGAATGGGGGCCAAGAGTCAGGGCAGAAAAGAAGACCAGCCTGAGAGTCA10.8264462809917356No Hit
AAGAGACAATTGAAGAAAAATTTGAGATTACAGGAACTATGCGCAAGCTT10.8264462809917356No Hit
CCCCTCAAGTTGTGGAGACGCATATAGACTGTTGAATACAGATTTTGCCT10.8264462809917356No Hit
ACCGAATATTCAATGAAGGTGCTTAATAATTCACACTTGTTTTTCTAAGC10.8264462809917356No Hit
CTATCGCAGTCCTCAAGAGCATGTCTCCTATTTCAAGAACACAGTATTTT10.8264462809917356No Hit
GAAGAAAAAACAAAGACTGAATAAGAGCGCCTATCTAATAAGAGCACTGA10.8264462809917356No Hit
TTTCTGAAGTGGACAGGGCGGAAGGTAGCATGTTTCAGCCTTTCGGGCTT10.8264462809917356No Hit
TCCAAAGAATGTCTTCATGCATTTGATTTCATCAAATAGTGGTATTACCT10.8264462809917356No Hit
GTGGTGGGGGGTAGGGGCGGGGGGTGGAGGAGGTGCCGGTCGGCGAACGG10.8264462809917356No Hit
GAACAACAACTCTTCCAGTGGTGTCACACCCATGAGCCTGTTCCTTCCAC10.8264462809917356No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG10.8264462809917356No Hit
GCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTCATTCCCTCCC10.8264462809917356No Hit
GAAGTCGACCCTCTCATAAATCCGGGAACTACAAACCATGCCCGCCAGCA10.8264462809917356No Hit
TACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATGTTT10.8264462809917356No Hit
TTGGGACCTTTACAAGAGAGATGCTAATGGGTCTGCTGACACTGCTGCGC10.8264462809917356No Hit
GATTGATCCAGTTGATAGTAAGCGGGAGAGACGAGCAGTCAATTGCTGAG10.8264462809917356No Hit
TATCTAATAAGAGCACTGCCATTAAATACGATGACCAAAGATGCAGATAG10.8264462809917356No Hit
GTATCTGCTTCTCAGTTCCAGGGTATTGGAGTCCATGGTTTCCACATTCT10.8264462809917356No Hit
TTCCCAATAGAGCCTTCCTCCACTCCCCTGGGTGACTCTCCGATTGGCCA10.8264462809917356No Hit
TGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCGAGGGATCAT10.8264462809917356No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA10.8264462809917356No Hit
GAAAACAAACCGGTATGGGTTCTTTATAAATGGAAGGTGTCATTTGAGAA10.8264462809917356No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG10.8264462809917356No Hit
CCCCAATACACTAATCAGGCAAGAAAACAGAATGGTCCTGGCTAGCACTA10.8264462809917356No Hit
ATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAG10.8264462809917356No Hit
GTGGTTTGGTTGCTGCTGGGGAGGGTCTGGAATGCAGTGTTAAGATACCA10.8264462809917356No Hit
GAAGCAGGCAGCGCCTCATTTCCATGCCCCATTTCATCTTGATCTTGGAG10.8264462809917356No Hit
CTAATAGCCCTACCTTTGATTGGTTTTGATCCCACAGCTTCTTTAACTCA10.8264462809917356No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC10.8264462809917356No Hit
GTCCTGCACACTTTCCCAATAGAGCCTTCCTCCACTCCCCTGGGTGACTC10.8264462809917356No Hit
GCTAAGTACGGTCTTGGGAGTCTCCCTACTGCATCTTGGACAAAAGAAAT10.8264462809917356No Hit
GCCCTCCTCAGTGAAAGCCCTTAGTAGTATCAAGGTGTCTAATCGGTTAA10.8264462809917356No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTGTCTTG10.8264462809917356No Hit
ATTCGACACTAATTGATGGCCATCCGAAGTCTGTTGGTCGCTGTCTGGCT10.8264462809917356No Hit
GGTTCATTCACTATGATAGAAAAATAGTCTGATGATTGGGTCCCAGAAAA10.8264462809917356No Hit
GATGAGAGCAGGGCAAGAATCAAAACTAGGCTTTTCGCTATAAGACAAGA10.8264462809917356No Hit
ATCCCAGGACCATGCGATAAAGACATGACAATAAGGCATCGGCTATAATC10.8264462809917356No Hit
GTTTCCTTTGGCATATCCGCAAGCGATTTGCAGACAATGGATTGGGTGAT10.8264462809917356No Hit
ACCCTCTGGAGCAAAACAAACGATGCTGGATCAGACCGAGTGATGGTATC10.8264462809917356No Hit
GGCGGGCGCTGTCCTCCGGCGGCTGTGGGCGTGTGCGAGCGCGCCGGAGG10.8264462809917356No Hit
GTAGGAGTCTATCGGATTCCTTTCGTCAGTCCGTTCGAGGCGAAGAGCGA10.8264462809917356No Hit
GAAGAAGTCAGTGAAGCGCAAGGAACTGAGAAGTTGACAATAACTTATTC10.8264462809917356No Hit
ATTCTGGAGGACGCCCCCACTCCCCCTCAGAATACCTGATGGGCCTCTTT10.8264462809917356No Hit
GGGTGTGGTCAGAAAGGTAGCGCGAAGTAGGTACAGATGCAATTGTCAAT10.8264462809917356No Hit
AGTAGAAACAAGCTCGTTTTTAAACTATTCGACACTAATTGAAGGCCATC10.8264462809917356No Hit
CATATACAACAGGATGGGAACAGTGACCACAGAAGCTGGTTTTGGTCTAG10.8264462809917356No Hit
GGATGATGATGGGCATGTTCAACATGCTAAGTAAGGTCTTGGGAGTCTCG10.8264462809917356No Hit
TTTATGTACACTCCCTTCATTATCTATTCAGTAGCCCAGCAGTGGGACAC10.8264462809917356No Hit
GATAATGAACCAAGTGGGTATGCACAAACTGGATTCCCAACAGCAATCGT10.8264462809917356No Hit
ACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGGTCATTGAAGCTTAA10.8264462809917356No Hit
GCACTTCAATACTTAATATCAGAGCATCCATCAGCACGAACTTAGACCGC10.8264462809917356No Hit
GTGTAAATTCTCCACTTTCCTTTGTATAATTTCTAGTTTAACGCATTGTG10.8264462809917356No Hit
GCTACAGGACATTGAAAATGAAGAGAAGATCCCAAGGACAAAGAACATGA10.8264462809917356No Hit
AATGAAGTGCGGGCAAGAATACTGACATCAGAGTCACAGCTGGCAATAAC10.8264462809917356No Hit
TGAGCTCACTCATACTTTATGACAAAGTAGAAATAAGGAGAGTTTGGCGC10.8264462809917356No Hit
AGTTAGTGGGAAGCAACATGAGCAAAAAGAAGTCCTATATAAATAAGACA10.8264462809917356No Hit
ACTAGGTACTAGCCATCCCCTCTCCCCCAAAGACCAGAAAATAGCTCCAG10.8264462809917356No Hit
CAATAATTGTGGCCATGGTATTCTCACAGGAGGATTGCATGATCCGGGCA10.8264462809917356No Hit
CCATAGCGATAAAAAAAGCTTGTGAATTCAAATGTTCCTGTCTTATTTAT10.8264462809917356No Hit
TGGATGCTCTGAAATTAAGTATTGAAGACCCGAGTCACGAGGGGGAGGGA10.8264462809917356No Hit
GTTCGTCTACAAATGGAATTTCTGAATGTACTGCCCCAGCCCTGCATCTC10.8264462809917356No Hit
GCTAAAACAGCCAAAACTTCTTTGTAGAACAAGAATTTTCACAGACTCTC10.8264462809917356No Hit
GCAGGAGGACTGGTTTGCTAATCTTGTTTTTTTGCATCATCAACTTGCTT10.8264462809917356No Hit
CATTGGGAAGTTTGGTTCATGTATTCTGATGTCCATGTCATCGCCGCACG10.8264462809917356No Hit
CTCACAAGAGGCAATGTAAGCTTTCCGTAGAGTGTCTGAGCCTCTGACAA10.8264462809917356No Hit
GTTAGGGGCGATCTGAACTTTGTCAATAGGGCAAACCAGCGACTGACCCC10.8264462809917356No Hit
GATTTGAGATAATTGAAGGAAGAGACCGAATCATGGCCTGGACAGTGGTG10.8264462809917356No Hit
GTCCTACACGCTTTCCCATTAGAGCCTGCCTCCACTCCCCTCGGAGACTC10.8264462809917356No Hit
AGTCCAGCCCCATGAGAAAGGCCGAAATCCCCATGACCTCCTGGCTTGGA10.8264462809917356No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT10.8264462809917356No Hit
CTCAAAAGGAGTGAAGGCCAAAAATGAACAATACTTGAGGACCACACAAC10.8264462809917356No Hit
GCCATAGGGGAGACACACAAATTCAGCCGAGAAGATCATTTGAGTTAAAG10.8264462809917356No Hit
GCAAGATCCCAATGATATTTGCTCCAATGCCGAGAGTATCAATTGAATAG10.8264462809917356No Hit
GTACCAGAAAAGCCAGTGAGAAGTGTTGACTAGAAAGCACTCACGGACAT10.8264462809917356No Hit
TACCCCAAGAACGGAGATGTCGCTGAGAGGGATAAGAGTCAGCAAAATGG10.8264462809917356No Hit
GAATCAATATCTTTATGACTGACAAAGGGATTCAGGGCATTACAAAGTCT10.8264462809917356No Hit
GCCGAAGATTTCAGTGAAACGCAAGGAACTGAGAAGTTGACAATAAGTTA10.8264462809917356No Hit
TAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAA10.8264462809917356No Hit
CCTCAGAAAAGGGAAAGTGGACGACAAACACCGAGACTGGTGCCCCCCAG10.8264462809917356No Hit
CAGTCTGGCCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTCCCAAA10.8264462809917356No Hit
CTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA10.8264462809917356No Hit
ATATACTGGTGGGATGGGCTCCAATCATACGACGATTTTGCTCTCATAGT10.8264462809917356No Hit
GAGTTCTCACTCACTGACCCGAGACTGGAGCCACACAAATGGGAAAAATA10.8264462809917356No Hit
ACCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC10.8264462809917356No Hit
ATATGATGCAATCAAATGCATGAAGGCATTCTTTGGCTGGAAAGAGCCTA10.8264462809917356No Hit
AAAGAAGAAGAAGTGCTAACTGGCAACCTCCAAACACTGAAAATAAGAGT10.8264462809917356No Hit
ATTCAATATGGAGAGAATAAAAGCACTGAGAGATCTAATGTCGCAGTCCC10.8264462809917356No Hit
GGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGA10.8264462809917356No Hit
GCCTGGTGCACGCCAGCTGTACCCGATTGATGGACCACGACCTGTGGATA10.8264462809917356No Hit
CTCTAAAACCCCTGCCTACTGCCTCTCCCCAGGGGACCCGGACAGGTCAA10.8264462809917356No Hit
CCTTGTTTTACGGACCAATTCTCTTTCTACCATGTACGCCACCATCAAGG10.8264462809917356No Hit
AGGGAAAGGGTACATGGGCGAGATTAAAAGAATGACGCTTAGAACACAAA10.8264462809917356No Hit
GTGTGGTGGTTGGTGGGGGGGTGGGATTGCGGCGTGTTCGGGGCGGGGGT10.8264462809917356No Hit
CTCCGCATGCTTGCGATATGTTCAATCGGGGCAAGATCTTGTCCTATGTC10.8264462809917356No Hit
GGCCCCCTCAAAGCCGAGATCGCGCAGAGACTGGAAAGTGTGTTTGCAGG10.8264462809917356No Hit
GGCACGGTGAGCGTGAACACAAATCCTAAAATTCCCTTAGTCAGAGGTGA10.8264462809917356No Hit
GTGAAAACCAGCTCAACCCTATTGAAGGACGACTGCCATAGGGAAAGTAA10.8264462809917356No Hit
TCATTGCCCTCTGGGCAGCTGTTTGAAATTTTCCTTTGAGGATATTGCAC10.8264462809917356No Hit
ACTTCAATGAATCCACAAATAAGAAAATTGACAAAATAAGGGCTGTACTA10.8264462809917356No Hit
GCCCAGCACAGTCTGCCCCATCGGGTGAATGTATAAAGAAGCTGGACTGT10.8264462809917356No Hit
ACTTTCAGCTGATCCCAATGATAAGCAAATGTAGGACCAAAGAAGGAAGA10.8264462809917356No Hit
AGTTTTTTACTCTAGCTCTATGTTGAAAAAATGACCATCGTCAACATCCA10.8264462809917356No Hit
GTTCAAGTCATCCTATCGTCAATGTTGCTAATAAGAAACTGATATATGAC10.8264462809917356No Hit
CTGTTCCAGCGAATGCGGGATGTGCTTGGGACATTTGCCACGGTCCAAAT10.8264462809917356No Hit
GCCTGCAGATACAGATATAGGTGCCATATGGGAGACAGCAAAAATCAGAC10.8264462809917356No Hit
GGAGGGAAGTCCACATATATTACCTAGAGAAAGCCAACAAAATAAAATCT10.8264462809917356No Hit
GGGGGGTGGGGGGGGGGTGGGTGGGGGGGGGGGGGGGGGGGGGGGGTGGG10.8264462809917356No Hit
GTATTCGTTGTTTCATACATGAATTCCGATTTCCCATTCATCGACGAACG10.8264462809917356No Hit
AGCAGCAGCCTGGGGTGTGGCGAGGTGCCCTAAGTCATAGTGTGGGAAGA10.8264462809917356No Hit
GAACCAAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCT10.8264462809917356No Hit
CTCCGTGCTTGGGGTCATGTCGGGCAGTATTCCGATCATTCCCATCACAT10.8264462809917356No Hit
CAGCAGGAAGGCAAGAGAAGAACGCCGCCCTCAGAATGAAGTGGATGATG10.8264462809917356No Hit
GGCATGTGAATTGACTGATTCAAGCTGGATAGAACTTGATGAAATAGGAG10.8264462809917356No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG450.002760009616.111113135-139
Produced by FastQC (version 0.11.5)