FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R1_D09-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R1_D09-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1067830
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA20130.18851315284268094No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC17830.16697414382439152No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT17120.16032514538831089No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG17040.15957596246593558No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA15060.14103368513714729No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT14570.13644493973759866No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT13840.12960864557092422No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA12940.12118033769420225No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA12890.12071209836771772No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT12100.11331391700926177No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA11990.11228379049099575No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC11090.10385548261427381No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA11050.10348089115308616No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC10950.10254441250011706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGAA5600.073.8379061
AGCGAAA1650.057.15471
GTAGAAA9000.049.9737241
CAATTCG5850.048.282399
GGTCGTT3950.045.8355677
AGGTCGT4000.045.2626236
CCTTATA8450.043.7543142
TTATACT9150.041.9522364
TCGTTTT4500.038.625919
CGAAAGC2450.038.4396483
TATATTC7000.038.29183
GCGAAAG2550.036.958182
CCCTTAT10050.036.8126141
TAGATAT2002.2555469E-1036.2219733
TTTAAAC8200.035.3385123
ATATTCA7400.035.2347454
ACAATTC8400.033.6252378
ATATAGT6700.032.4817161
TAGAAAC15150.032.0379143
GTCTAAG7300.031.7994561
Produced by FastQC (version 0.11.5)