FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R1_D07_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R1_D07_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2046
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACAGAATGTGGCTTAAACATGGGATCATAGGTCTAAGCGGGCA90.43988269794721413No Hit
GTCAAATGGAAGAGAGAGGGTTTGTCCTTCGGTTTCTAGATGGTTCTGCA50.24437927663734116No Hit
CCCTAAGAGGGGCTGAGTAGAGTCACAGCGGAGGTAATGCCCTGGACCTG50.24437927663734116No Hit
GCCCAGCACCTTCCCGTAAAGGACCTGATCACTGGTGATAGACGGGGGCC50.24437927663734116No Hit
GTATGGGGCACTCCGGGCCGAGGGGGCAGCAGGCTTGGGGCCTTCGGCCA50.24437927663734116No Hit
GTACAGGCTGGGGGCGAAGGCTCTGCTTTCACACCCGGGGGCGTTCCTCA50.24437927663734116No Hit
CTCCCGCGCTGTGTCCCAGCTTGTTAGATTTCTAGCATAAAAGCCTTGTG40.19550342130987292No Hit
CCCTTACCCACTACACCATCTTCTGGACCAATGCTCAAGACCAGTCCTTC40.19550342130987292No Hit
GTTAGAGCTTGTGGTAAAGGCAGCTATGGGAGACTGGGCCTTGGAGATTC40.19550342130987292No Hit
GCTCAAGACCAGTCCTTCTCCACTGTCCTGAATGCCTCCTCCCATGGCTT40.19550342130987292No Hit
TTCTAGCACCGTGATCTTGGTGATGGGTGTCATGCCGGGGTCCCGAATGC30.1466275659824047No Hit
CACATATACACAGAATGTGGCTTAAACATGGGATCATAGGTCTAAGCGGG30.1466275659824047No Hit
ATGCAGTTCCGTGCATTCCACTCAGATCTCAACATTGTACCTTTCCAGTT30.1466275659824047No Hit
AGTGAAGACAGTAATGCCTTTCTTCCCTTGCAGGATTTTGCATGGATGAA30.1466275659824047No Hit
TACTAACAGAGTGTATAAACCTAAAGTTGTTGAAGCTTTACAAGGAATGT30.1466275659824047No Hit
GGGGATAGGGAAGATTCTAGTTCTGTTGCTGAACTCTGGTGGATTAATTT30.1466275659824047No Hit
CCTTAGGGAAGGGATTGGCCACCGGCATCCGGGCATCCCGCAGCTCTTCG30.1466275659824047No Hit
GTCCAGTTGTGTCACAAACTCACTACATGACTTCAGGGAAGTCACACCCT30.1466275659824047No Hit
GTGCATGTCACTGCGTGTCTCCTCTGGGGCCGCCTCCTTGGGGGAGTAGA30.1466275659824047No Hit
CTCCGGGCCGAGGGGGCAGCAGGCTTGGGGCCTTCGGCCACACCGTAGTG30.1466275659824047No Hit
GTGATAGACGGGGGCCGGGGCTGGTTGGAAGGTGCTCTTGGGTCCCCTTG30.1466275659824047No Hit
GTGTATATGTGCGTGAATCTGTCATTCTTCAGATATTAGACAATGTAAAG30.1466275659824047No Hit
GAATAAGCTTGGGATATTTCTGTGTTGAACTATTTCCATGTCCCAGTTTC30.1466275659824047No Hit
CCCCAGAGCTGCATCTAAAGCACATTGGCAAGACCTGGGCCCAGCTGGAG30.1466275659824047No Hit
CTATTGCTTTGTCTAAAGATGGGAGAACTGTATGGTCTTTTGGAGGAGGA30.1466275659824047No Hit
GCCATGATGTCCTGGGGCATCAGCATCCGGCTGTTCAGAAACTCTTCACG30.1466275659824047No Hit
GTCCTGAATGCCTCCTCCCATGGCTTGGTCCTCCACAGCTTGGAGCCTGC30.1466275659824047No Hit
GGATAGAATGCTTAACAGGAGCTGGAAACCCTGATGTTGGAAGAAGATTC30.1466275659824047No Hit
GATACTAACAGAGTGTATAAACCTAAAGTTGTTGAAGCTTTACAAGGAAT30.1466275659824047No Hit
GCCTCGCCTAGACAATTATGTTCTGGATACGGGAGGGAGATGTGGATAGA30.1466275659824047No Hit
GGAGAAAACAGAGAGGAGACACGGGCCTCCGAGTGAGAACTGGTGGACAG30.1466275659824047No Hit
ATCTTATACCATGAGCATTTTCCCACAATCATGTGCTATCCCTCCCTTAC30.1466275659824047No Hit
GGTTTATGCTTGGGGCTGTGGAGCTTGTCTAGGTTGTGGTTCTTCAGAAG30.1466275659824047No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)