FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R1_D06-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R1_D06-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000865
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC12010.1199962032841592No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT11190.11180329015401677No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT10520.10510908064524187No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATCG350.003580457562.0816355
AGCGAAA2500.058.067621
CGAAAGC2950.049.1090283
GCGAAAG3400.042.64342
CAATTCG2650.041.0055059
AGTAGAA6400.040.8287931
GTAGAAA7350.036.5391461
TTATAGG2351.2478267E-930.8870321
GTCTAAG6750.029.033811
ACCTATC2354.332651E-827.7427648
ATATAGT5500.027.7140881
CTGATCG2654.5511115E-927.362986145
ATATTCA4300.026.9501694
GGATAGC4150.026.1790055
GTATTAA1958.675655E-626.0559831
ATAGTGG5350.025.7248693
TGTATAC1701.0318945E-425.5860392
CTTAGAC6800.025.5655823
GATAGCA4250.025.561756
TCTAAGG7750.025.2558962
Produced by FastQC (version 0.11.5)