FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R1_D05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R1_D05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTCAGAGACTCGCACCGTGTTACCATTCCATTCAAGTCCTCCGATGAG14.545454545454546No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA14.545454545454546No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATATTCGAGA14.545454545454546No Hit
TTTATGTACACTCCCTTCATTATGTATTCAGTAGCCCTGCAGTGGGACAC14.545454545454546No Hit
CACTTAGGGACAGCATGGATCCTGGATCCTTCGATCTTGGGGGGCTATAT14.545454545454546No Hit
GTGGAGATCTTGGATGATGACAATTTCCTGGGGGCAGAAAATGCCTTTAA14.545454545454546No Hit
GTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTATC14.545454545454546No Hit
CCTTTGGCATATCCGCCAGCGATTTGCAGACAAAGGATTGGGTGATGCCC14.545454545454546No Hit
GGAAATGCCTCAAGAGTTGGTGCATGGGCTTCAGGCGCTGGTTTGCCCTA14.545454545454546No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT14.545454545454546No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC14.545454545454546No Hit
AACCGGCCCCCCTGCTATTCTAAGTTACTCAACCCTGACGTAGACAGCAA14.545454545454546No Hit
CGGCCAAAAACTGTTACTCGATAGATAAAACGACAATATTTGTGGGAACA14.545454545454546No Hit
GTCTGCTGACACTGCTGCTCTTCTTACTATGTTTCTAGCAGCGATAATCA14.545454545454546No Hit
AGTCAAACGACAAGAGAAAGGCATAAATCCCAATTACCTCAGGCCTTGGT14.545454545454546No Hit
TTAATCAAGATTGTTTTCAGTGTTGTAATTCTAGGTGGGTAACCCTTCAC14.545454545454546No Hit
GTGTAATCTTTAACCGATTAGGGACCTTGATACTACTAAGGGCTTTCACT14.545454545454546No Hit
GAATAGGACTGGCCCAACCACAAGTCCAGTTCATTCCGCTAAGGTATATA14.545454545454546No Hit
GTATTCATCTACTCACATTTTGCTGACTCTTATCCCTCTCAGCGAAATCT14.545454545454546No Hit
GCTTAGTGACACCTCCTTGGCCCCATGGAACGTTATTTCTCTTTTGAGCG14.545454545454546No Hit
CAGTATATTTTCAGAGACTCGAACCGTGTTCCCCTTCCATTCAAGTCCTC14.545454545454546No Hit
GTATGCTAGAGTCCCGTTTTAGTTTCATTTCCAACACTACGGCGCCTTGC14.545454545454546No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)