FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R1_D05-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R1_D05-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences853541
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG13870.16249951671917343No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA11750.13766181120766313No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA10730.12571159440495536No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA10630.12454000452233696No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT9430.11048092593091603No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG9370.10977797200134498No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC9300.10895785908351209No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA8760.1026312737173727No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC8690.10181116079953981No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1250.075.468481
CAATTCG5250.056.5774969
AGTAGAA5100.055.491531
CGGTATG400.0060681854.33519
CGAAAGC2100.044.858533
GGTCGTT3000.043.465537
TTTAAAC7400.040.1488533
GTAGAAA7250.040.0175442
AGGTCGT3300.039.5118066
ACAATTC7900.039.4330678
AGAATCG750.001489482138.6337666
TCGTTTT3450.037.798339
GCGAAAG2750.034.2877582
GACAGTA2154.9112714E-1033.6942147
GTGGTAT4650.029.598456
CAACTCG1000.00607139429.00251145
GGTCTAT2654.5129127E-927.3833391
ATATAGT5650.026.9713761
TATATTC6200.026.8817253
CTTAGAC6200.026.8817253
Produced by FastQC (version 0.11.5)