FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R1_D04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R1_D04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1234005
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA89690.7268203937585341No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT69410.5624774615986159No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT48200.3905980932005948No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT39690.3216356497745147No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG33760.27358073913800995No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG29300.23743825997463547No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC28890.23411574507396649No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC27240.22074464852249384No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT27060.21928598344415134No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG26480.21458584041393675No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG25770.20883221704936367No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG25020.20275444588960337No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG24930.2020251133504321No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA22230.1801451371752951No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC22220.18006410022649827No Hit
CCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGG21830.1769036592234229No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT21670.17560706804267406No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC21510.1743104768619252No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG21150.17139314670524025No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG19330.15664442202422194No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG18940.1534839810211466No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA18550.15032354001807124No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG17930.1452992491926694No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA17890.14497510139748218No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG17320.14035599531606438No Hit
AAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAG17180.1392214780329091No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG17140.13889733023772188No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA17000.13776281295456663No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA16980.13760073905697304No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA16760.13581792618344335No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC16610.13460237195149127No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG16320.13225230043638397No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA16130.1307125984092447No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG15680.1270659357133885No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA15440.12512104894226522No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC15420.12495897504467163No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC14020.11361380221311906No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG13300.10777914189974919No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG13230.10721188325817155No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT13100.10615840292381312No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA12880.10437559005028343No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA12860.10421351615268983No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC12740.10324107276712816No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG12740.10324107276712816No Hit
GAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACT12700.10291692497194095No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT12690.10283588802314417No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA12650.10251174022795694No Hit
CACAGAGACTGGTGCACCCCAGCTCAACCCGATTGATGGACCACTACCTG12640.10243070327916014No Hit
ACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTG12520.10145825989359848No Hit
GTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGAGAATAGAACGATTC12500.10129618599600489No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTTA100.0070855645145.03761145
AGTAGAA8250.0115.151071
GCAGGTA3650.0105.237222
GGTACTG4300.090.959415
GTAGAAA11650.082.117252
CAGGTAC4700.081.6870353
AGCGAAA1500.077.353391
AGGTACT5300.075.1669164
CGAAAGC1600.072.4394453
GACAGTA4600.070.8531957
AGCGAGC651.3958197E-766.94044145
GCGAAAG1750.066.262582
ATATTTA502.0207721E-457.9398166
TATATTC5150.053.450473
ACTGATC7350.053.214358
CCTTATA30500.051.326342
CCCTTAT30250.051.302561
TTATACT30950.050.5513764
AGGTCGT3950.049.505546
GGTCGTT4000.048.8887027
Produced by FastQC (version 0.11.5)