FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R1_D04-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R1_D04-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences857458
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGCG300.001940299872.456157
AGCGAAA1650.048.3915861
TAGACCT2600.039.0148474
CGAAAGC2057.2759576E-1238.8902473
GGTATAG750.001474513238.713271
CAATTCG2151.2732926E-1137.068429
TATACGT800.00203433736.257682
GTTACGC1001.3938514E-436.238643
GCGAAAG2302.5465852E-1134.681262
ATCCCGA2455.638867E-1132.5313266
TATCCCG2507.094059E-1131.8807035
ATATAGT5400.030.9168451
GTAGAAA5400.030.9168451
CGTACCA1204.0733695E-430.1882979
TCCCGAC2701.72804E-1029.5191697
ATATTCA4500.028.9824564
ATAGACC5050.028.703873
GTGGTAT5600.028.4649126
TTACGCA1306.505164E-427.8677484
GGTCTAT3453.6379788E-1227.3517651
Produced by FastQC (version 0.11.5)