FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R1_D01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R1_D01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCATGGAAAAGAAAATAGTAATGAACGCTACCGTCAGGGTAATCTTTAA12.380952380952381No Hit
AATCACAAGTTACATTCAGTATTTTTGACTTCTTATGTCTTCAATGAGTG12.380952380952381No Hit
GTATAGTTGTTCTTTTTTTCTGCTTTGTCTCTGCAAACATTCCTTTTTGT12.380952380952381No Hit
ATATGCGTCTCCACAACGGGAGGGGTTTTCGGCTGAATCTAGAAAATTGC12.380952380952381No Hit
CCACATATATTACCTAGAGAAAGCCAACAAAATAAAATCTGAGAAGACAC12.380952380952381No Hit
TGCAGAGAGATGCAAGTTAAAAAGAAGGGCTATCGCATCACCTGTGATGC12.380952380952381No Hit
GACAACAAATCAGACGGAGAACAATGGCCATCATTCAGTTCAAGTCAACA12.380952380952381No Hit
GGCAATAACTGCTAATAAAAATAAGCAAAAAATCCCAAATCAAGTTATAT12.380952380952381No Hit
GAATTGTTTACATAATAAATCTCTTTATGACTGACAAAGGGATTAAGTTG12.380952380952381No Hit
TTGCAGTAGAGAGCACATTCTGAGATCGATTCCGGTGCGAACAAGCTCTC12.380952380952381No Hit
GTATTCCTCTACTACCATTTTGATTACTATTATCCATATAAGCTAAATAT12.380952380952381No Hit
GATCAAACACAATCAAAGGTAGTGCTATTAGTATCAGATGGAGGACCAAA12.380952380952381No Hit
AAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGT12.380952380952381No Hit
GGACCCTGGAACCTTCGATCTAGGGGGGCTATATGAAGCAATCCAGGAGT12.380952380952381No Hit
CCCATAATGTTCTCACACAAAATGGCAAGACTAGGGAAAGGGTACATGTT12.380952380952381No Hit
ATGTTACATTTACCCAAATGCAATGGGGCTACCCCTCTTAGTTTGCATAG12.380952380952381No Hit
CTTAAATATAAACACTGCCTGTTCATCCGGCTACTGGGAGATACATTGTT12.380952380952381No Hit
CCCTACTGGGAGACCAGACTGTTAAAGCTTTTCGCAAATGCTACTAGCTA12.380952380952381No Hit
GGGTTCACGCTAAAAGTGCCAAGTGAGCGAGGACTGCAGCGTAGACGCTT12.380952380952381No Hit
CCTCAACCCTGATCCTCTCACATTCACAGTCAATGATGAAAATTGCATCC12.380952380952381No Hit
GCCCTAGACACAATGCCCTCCAACATGCTAGAAATTAAAACCGGTCACAT12.380952380952381No Hit
CCAGTGAATGAAAAGATGTGAATGTGTGTCTTCTCAGATTTTATTTTGTT12.380952380952381No Hit
CAAGGAGGCAATTTTGCATTCCTGGCGCTGTTCTTGCTTCTCTTTTGGTT12.380952380952381No Hit
GCTTTGGAGTGTCTGGAGTAAATGAATCCGCTGACATGAGTATTGGAGTA12.380952380952381No Hit
CAGAGAACTCTTCTTTCTTGATCAGTCCAGACTCGAAGTCGACCCTGGCA12.380952380952381No Hit
GGGTGAATCAATAATTGTAGAATCTGGTGACCAGAATGAACTATTGAAGC12.380952380952381No Hit
CTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATGGG12.380952380952381No Hit
ATGCACTATTGAAGCACCGATTTGAGATAATTGAAGGAAGAGGATGATTG12.380952380952381No Hit
GATAGATACAATTTTTTAGAATTCATAGGAGATCTTATGTGGCAAATCTG12.380952380952381No Hit
AACCATGAGGGAATACAAGCAGGAGTGGACACATTCTACAGGACCTGCAA12.380952380952381No Hit
GAGACTGGTGCACAAAAGATCAACCCGATTGATGGACCCCTACCTGAGGA12.380952380952381No Hit
ATTGGAAAGGATTCAGGTGCATTGAATTAAGATACAGATGAAGGCAAATC12.380952380952381No Hit
CCTATATGTGAGAACCAATGGAACATCCAAGATAAAGATGAAATGGGGCA12.380952380952381No Hit
TTATAGGTGGCAGGCACACGAGGTTTGTGATCCAATCGGGTTGTTTTCTG12.380952380952381No Hit
GCATGAGCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGAA12.380952380952381No Hit
ACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTGAT12.380952380952381No Hit
CTTTTGATTCTTGCCCGGCGCTCAACCTATAGGGTGTAGGCCGCTTTGGT12.380952380952381No Hit
GGGCCGAAGGTACCATGTTTCAACCTTTCGACATTTTCGAAATAAGTTTT12.380952380952381No Hit
AGTCAGTGCTAGTCAACACTTATCAATGGATAATCAGGAACTGGGAAATT12.380952380952381No Hit
TTGATGGACAATTGAAGAGCCATCTGGGCCGTTGCAGGTCCAAGGGCATT12.380952380952381No Hit
GATTAGTTTTGACGTCTTAGTTATTCAAAGGTCGAACAGTACGTCATGAT12.380952380952381No Hit
GGCCACCTCCAAACACTGAAAATAATAGTACATGAAGGGTATCAAGAATT12.380952380952381No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)