FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R1_C11-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R1_C11-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences321354
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAGAGAATTACTTAATATGTTAGTAGAGTGGATATTAGATGGTGGAG8990.27975379176857923No Hit
TGTTAGTAGAGTGGATATTAGATGGTGGAGACAATTAGTCGACCGCTATG8420.26201634334721213No Hit
CTATTATTGTTCCCATTTTACAGTTGAGAAAGCCAAGGCTCTGAGAAGCT7990.24863546120477728No Hit
AAGCAGGACCAATCAGAGAAGAGCCCGTCCTGCTTTGGTTCCCGCCCACA7890.24552362814839712No Hit
TCTTTATGCTCCCCAGAAAACCATTTACTAATGCCCATGCTTACCTCCTA7660.23836641211872267No Hit
ATATTTATCTATGGCTTCATTTTAGTGTCAAAACTCACACACGCGAGGAA7490.23307629592287635No Hit
GCTATTAAGGGTAGGCAGGAGAAGATGCATTTAAGACTTACTTAGGAGGT6960.2165835807240613No Hit
TCAGAGAAGAGCCCGTCCTGCTTTGGTTCCCGCCCACAGTGTCAAAAGTC6790.211293464528215No Hit
GCATAAAGAAGGATATCAAAATAGGGGTGGCGAAGGAACTCGTGTTGCAA6470.20133559874779836No Hit
GTACTATTATTGTTCCCATTTTACAGTTGAGAAAGCCAAGGCTCTGAGAA6430.2000908655252463No Hit
AAATTAGAGGATGTAAATTGATATTTATCTATGGCTTCATTTTAGTGTCA6430.2000908655252463No Hit
ATCTATGGCTTCATTTTAGTGTCAAAACTCACACACGCGAGGAAGCGTGT5860.18235341710387923No Hit
GAGAAGAGCCCGTCCTGCTTTGGTTCCCGCCCACAGTGTCAAAAGTCATA5470.17021726818399646No Hit
CCCTGAAAGTGTGCCTTCTCCTCTGGGCCTCATCACAGTTCACGGTGCCA5470.17021726818399646No Hit
CTCCAGAGGCGCCCTACTCACTCGCTTTCAGGTTTGTCCAGCTAGCCAAA5360.16679425182197827No Hit
AATCAGAGAAGAGCCCGTCCTGCTTTGGTTCCCGCCCACAGTGTCAAAAG5350.16648306851634023No Hit
CCTCTGGGCCTCATCACAGTTCACGGTGCCAGTACTCTCAGGTGACTGGT5340.1661718852107022No Hit
ATATTAGATGGTGGAGACAATTAGTCGACCGCTATGGTAATCTGGCTAAA5260.16368241876559805No Hit
GGGTGGAGGTGGGATGGAGGGAAACATGGTGGGTGAGAAAAAGATTCGAT5260.16368241876559805No Hit
GTGTCAAAAGTCATAACTCGAGCGAGCAGATAAAGTTTTCGCGAGAGCGT5180.1611929523204939No Hit
AAATAGGGGTGGCGAAGGAACTCGTGTTGCAAGCCAACTCTGATTAACTG5120.15932585248666578No Hit
GGTTAGGACATCTGGTCTGTGGGTCTGGACTCCGAGTGCCAGGGTCTGAG5000.15559165281900958No Hit
CTATGAGCCCAGGCGGTGAGTACTATTATTGTTCCCATTTTACAGTTGAG4800.14936798670624918No Hit
CCCCAACCCCACGCACTTACTGTGAGTTGTTCCTCTACTGGTGTCCCTGA4780.14874562009497316No Hit
ACTCTACTCTTCCCTGCCTCCCACTCCATTGCTTTGGTGTAGTTCACAAA4770.14843443678933513No Hit
GTCCAGGAGGTCTGTGCTCCAGGAGGTCTGTGCTCCATCTTCACGGAGGA4710.14656733695550703No Hit
CACTTACTGTGAGTTGTTCCTCTACTGGTGTCCCTGAAAGTGTGCCTTCT4680.14563378703859295No Hit
GTGCCAGTACTCTCAGGTGACTGGTCGGAAATCAGGAGTCCCCCTCAGAC4680.14563378703859295No Hit
CCCCTATTTTGATATCCTTCTTTATGCTCCCCAGAAAACCATTTACTAAT4430.13785420439764245No Hit
GTCTAAAACCTGGTGCTAGGTCACAGAGCTTGTCACAAGAGAGGCAGGAT4390.1366094711750904No Hit
GTCATAACTCGAGCGAGCAGATAAAGTTTTCGCGAGAGCGTATGATAAAT4270.13287527150743417No Hit
CTTAATAGCTGTGCCCGAAATATGAAACTCTTGTTTTATTTTAGCCAGAT4180.130074621756692No Hit
GCTTTGGTTCCCGCCCACAGTGTCAAAAGTCATAACTCGAGCGAGCAGAT4170.12976343845105398No Hit
ATTGAGTGTTGACCGCGAGACAGAATGTCTGCAGAAGGTGGGAAGATTGC4170.12976343845105398No Hit
GGATATTAGATGGTGGAGACAATTAGTCGACCGCTATGGTAATCTGGCTA4170.12976343845105398No Hit
ATGGTAATCTGGCTAAAATAAAACAAGAGTTTCATATTTCGGGCACAGCT4160.12945225514541597No Hit
CCGTAGAAGCTCCTGAAGAATGTCTTTCAGTCTCTGTCTTACTGGACCAC4140.1288298885341399No Hit
GAGGGAAACATGGTGGGTGAGAAAAAGATTCGATGAAAATTAGAGGATGT4120.1282075219228639No Hit
GCTTTGGTGTAGTTCACAAATCGTTTATGGTCCTTTTTCCCACTGAACTC4050.12602923878339775No Hit
GAAACATGGTGGGTGAGAAAAAGATTCGATGAAAATTAGAGGATGTAAAT4040.12571805547775972No Hit
CTCCATTGCTTTGGTGTAGTTCACAAATCGTTTATGGTCCTTTTTCCCAC4010.12478450556084567No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT3980.12385095564393162No Hit
GGCATGGAGTGTGGAGAACACGCAAGCATACTGGGACGATTGAGTGTTGA3930.12229503911574152No Hit
ACTCTCAGGTGACTGGTCGGAAATCAGGAGTCCCCCTCAGACAGAGGTGG3890.12105030589318945No Hit
GCTCTATGCTTCTGTTACTTGAGCAAACTTGTTGGGAGAGTGTTTTATGG3860.12011675597627539No Hit
GAATTACTTAATATGTTAGTAGAGTGGATATTAGATGGTGGAGACAATTA3800.11824965614244728No Hit
GACATAATGTGAGTAAAGCCCTCATCACCTGCATGGCTGCTATGATAAGC3790.11793847283680925No Hit
GATGTAAATTGATATTTATCTATGGCTTCATTTTAGTGTCAAAACTCACA3730.11607137300298113No Hit
ATCTGGAAGTCACGGTACAGAGAATTACTTAATATGTTAGTAGAGTGGAT3710.11544900639170509No Hit
CTATGGTAATCTGGCTAAAATAAAACAAGAGTTTCATATTTCGGGCACAG3710.11544900639170509No Hit
TATTTGGGGGGCGGTATGCGGAGATTTATTAAAATTTCAAATTTATCACG3620.11264835664096293No Hit
CATCAGGAGGTTCCATCCAAAGTTCAAGGTTTCTCCTTCCATGAAATGTC3610.11233717333532489No Hit
CTTGTTGGGTGGGTGGTTGGATGGGTGGATGGACGGATGGACAGACGCTT3590.11171480672404886No Hit
CTCCAGAAACAACGCACTTCTTTCTCAGAGAGGTCTAAAACCTGGTGCTA3580.11140362341841084No Hit
ATTTAAGACTTACTTAGGAGGTAAGCATGGGCATTAGTAAATGGTTTTCT3570.11109244011277283No Hit
CTACTAACATATTAAGTAATTCTCTGTACCGTGACTTCCAGATAGAACAA3540.11015889019585878No Hit
CCCTCAGACAGAGGTGGAGGTGGCAAGCACGCGGCACCTACTCTACTCTT3530.10984770689022076No Hit
GTCCAAACACTTGTTGGGTGGGTGGTTGGATGGGTGGATGGACGGATGGA3480.10829179036203065No Hit
GTTTAAGGACCTTGGTGCTTCAGAATTACCAAAAGATTTTAGGACAGGAA3470.10798060705639265No Hit
GATAAAGTTTTCGCGAGAGCGTATGATAAATTCCAGCGAGCAGATCCAGT3390.10549114061128848No Hit
AGTAAATGGTTTTCTGGGGAGCATAAAGAAGGATATCAAAATAGGGGTGG3350.1042464073887364No Hit
GTCAAAAGTCATAACTCGAGCGAGCAGATAAAGTTTTCGCGAGAGCGTAT3280.10206812424927028No Hit
GTCCTAACCAGGCCATAGGCCAAATGGGGCCTATGAACATATTTGGGGGG3280.10206812424927028No Hit
GATGTGAGACGTAACTCTGAATTGTTCTATCTGGAAGTCACGGTACAGAG3250.1011345743323562No Hit
AAGTAGGAGGGCATGGAGTGTGGAGAACACGCAAGCATACTGGGACGATT3230.10051220772108017No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCA202.1400283E-6144.841134
CTTACGC256.488417E-6115.890923
TACGCAC256.493441E-6115.8728945
AGCCCTG301.6065216E-596.560747
TACCCTA259.39276E-486.9723362
TACCTTA259.421855E-486.904677
CGAAAGC3650.083.3462143
GCGAAAG3900.078.05212
GACCAAT2000.076.041597
GGACCAA2000.076.041596
CTTCCCG406.661653E-572.48824145
TTGTATA406.661653E-572.488241
ACTTACG406.666797E-572.476952
ACCCTAC300.001941775372.431833
ATACCCT406.6925626E-572.420568
AGCGAAA4350.071.655041
GAAAGCA4350.069.9232944
ACCAATC2250.067.5925148
CCGGAAT1009.094947E-1265.1886443
ACCGTAC902.8194336E-1064.4341
Produced by FastQC (version 0.11.5)