FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R1_C09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R1_C09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCGGGGTCCCCACTAGACCCGGGCACCTTTCCCCGCACTGCTGTC11.0204081632653061No Hit
GTGTATACTCTGCCTGAACGTAATAATAGCAGATATATTGGTGAACAAGG11.0204081632653061No Hit
CTGTTGGGTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAA11.0204081632653061No Hit
GGCCCGGTGTGGTGTCATTACCAACTCTACGTCACCTTGCCCACTTACCA11.0204081632653061No Hit
CAGTAAACAGAACACACCAAAACTCAGAAAAGGGAAAGTGGACGACAAAC11.0204081632653061No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC11.0204081632653061No Hit
GTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACCA11.0204081632653061No Hit
TCATACCACTATCTGCAACCCTGATCCGCTCACATTCACAGTCAATGAAT11.0204081632653061No Hit
GGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.0204081632653061No Hit
ACCGAAATCAGATAATTGAAGGAAGAGACCGAATCATGGCATGTCCAGTG11.0204081632653061No Hit
CAAATGGAAGAGAGAGGTTTTGTCCTTCGGTTTCTAGCTGGTTCTGCATC11.0204081632653061No Hit
ATTTCTTCTCTTATAGTGAAAACCCTATTGTTGATTATTGCCCTGCTCTG11.0204081632653061No Hit
CTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCTTTAACTCA11.0204081632653061No Hit
TCAGAGAACTCATCTATCTTGATCCCTCCAGCCTCGAAGGCGACCCTGGC11.0204081632653061No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCCAT11.0204081632653061No Hit
GGCAGAGGAAGAGGGAGAAGGAGAATCCCCAGGAGCAAGGAACCTGATGT11.0204081632653061No Hit
TGGACCTTATGTAGCAATTAAGAGCACTTAGACCCCCAGTCCAAGATTAG11.0204081632653061No Hit
GAGAAGTTTTGTCATTCGGTTTCTAGATTGTTATGCATCTTTTGAATTTA11.0204081632653061No Hit
CCTCTACTGGTGTCCCTGAAAGTGTGCCTTCTCCTCTGGGCCTCATCACA11.0204081632653061No Hit
CCCTTGGGTTAAGTGCAACACTTCAATATAAACACTGCCTGTTACGCCGG11.0204081632653061No Hit
ATCTATTATGGAGACAATCGGTTTGACTCTGTGAGTGAGTCAGGAACGGC11.0204081632653061No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGCAACAAATCTTG11.0204081632653061No Hit
CTAATCCTCAACCCTATTGCTGCCTTGCATATTTCAACCGCTTGTTCCTC11.0204081632653061No Hit
GTTCTTTCTCAATGTGACTCCAAGACTCATGTAAGCTTATGCATTTAATA11.0204081632653061No Hit
ATTGCAGCCCCGAGTCACGAGGGGGACGGATTAGCACTTTAAGATGCCAT11.0204081632653061No Hit
GGTCTCATTTGAGAAATGATACTGATGTCGTGAACTTTGTAAGTATGGAG11.0204081632653061No Hit
GATTACAAGCAGGAGTGGACAGATTCTACAGGACCTGCAAGTTAGTGGGA11.0204081632653061No Hit
NNGGGTTGCAGAGGAGGCGCTGGCTGAAGCTTCTTCCTTCTCTCCCGCCT11.0204081632653061No Hit
CAATAGAGAGGAGGGTGCTTTCTGCGTTTGATGAGAGAAGAAATAAATAC11.0204081632653061No Hit
CATGAATATGGCACACTTTGTTTATCCATTCTCTAGTTGAGGGATATTTG11.0204081632653061No Hit
CTGGCACACCTGGAAACTGGGAGGGAGCCCCTGGAGTATTTCCTCGCAGG11.0204081632653061No Hit
ATTTTCACCTCTGAGGTTTGGAACCACCGGGAAAGTGGGGCTGGTAAGAG11.0204081632653061No Hit
GCCATGTGGACAATTCCAGAGACACGGAATAAAGTTTAGAAGCCACCGTG11.0204081632653061No Hit
CCCTTATAATGTAGATACTCCATACACCCAAGCAACAGGAACAGGATACA11.0204081632653061No Hit
GGGCGGGGGGGTTGGGTGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGG11.0204081632653061No Hit
AAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCGTCATATA11.0204081632653061No Hit
ACAACGCTCTTTCAAAGAAAAAGGAGAGTAAGCGACAACATGCCCAAGAA11.0204081632653061No Hit
GTAGACATATGCAAGGCATCAATAGGGTTGAGGATTAGCTCTTCTTTCAG11.0204081632653061No Hit
CCCTCATGGTTTGGTGCATTAACTATGAGAGCAAAATCGTCGGATGATTG11.0204081632653061No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCGTGTTGGGAAACAAATAGTG11.0204081632653061No Hit
GCATTCTGGGTGTTGGGCTGGGTAAGCAGAAGCCAGGGTATCAGCCAGAG11.0204081632653061No Hit
GAAGCATTTCTCAAAAGACACCTTCCTTTTATAATGACCACATTCAGGGT11.0204081632653061No Hit
CAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATGTTGTGAGAAAG11.0204081632653061No Hit
GTTGTCTCTTACTATCCTTTTTCTTTGAAAGTGTGTTGTTATCTCTATTT11.0204081632653061No Hit
GTCCTTACGAATCAACTGTACCGGCGTCTGGTTGCCTTAGGGACAAGAGA11.0204081632653061No Hit
AAGTACTAAATGTCTATGCTTGCTAGCATTACTTCTGCTATTTGTGTTAG11.0204081632653061No Hit
ATCATTTGAAGAAAACTGGCCCCATCTTGTGGAAAGCTATCCTCTTGGAA11.0204081632653061No Hit
GAAAACCACACGACTCCGAAAAGGGACAGTGGACGACACACACAGCGCAT11.0204081632653061No Hit
AGCTATCCCTTATAGGAAAAACCTCTAACTTCGGTTCATTAACACTACCT11.0204081632653061No Hit
GAATAAAAGAACTGAGAGATATAATGTCGCAGTCCCGCACTCGCGAGATA11.0204081632653061No Hit
GGATTCCTCTGTCAAGATCCATTCCACGATTTGTTTCCCAACAAGATTGT11.0204081632653061No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGGACACATCAG11.0204081632653061No Hit
GCATTGAGGGCAAGCTTTCCCAAATGTCAAAAGAAGTGAACGCCAAAATT11.0204081632653061No Hit
CAATTGGCTTGTTCTCTTCATGTTCTTTGTCCTTGGGATCTTATCTTAAT11.0204081632653061No Hit
AATTTCTGGCCATACGAATTCTTTTGGTCGCTGTCTGGATGTCAGTAAGT11.0204081632653061No Hit
CCTTAGGGTAATGAACAGTACTTGTTGTTGGGCCATTCCTATTCCACCAT11.0204081632653061No Hit
ATAGGAGACCGGTTTGAATTTCTAGCATGGTGGAGGCAATGGTGTCTAGG11.0204081632653061No Hit
TTAGCCTTCTCTCGTTTTGCAAGATTGCTCAGTTGATTGATGCTTAATGC11.0204081632653061No Hit
CCGTTTTAGTCGAGATCGTCCATACACCCATGGAAGTGGATGAGCATATT11.0204081632653061No Hit
ATTTATGTACACTCCCTTCATTATCTATTAAGTAGCCCTGCAATGGGACA11.0204081632653061No Hit
ATATAGTGGAATTCCCTCCAACTAGTGAATCGGGTCTTCATTAATTAAAT11.0204081632653061No Hit
GTGTTATTTCAATTAATTCCCACAGGAACTCTATGAAGAAGGTCTATTAT11.0204081632653061No Hit
TTCCATCTCCCAGCTGGGGAAACCGAGGCACAGGGAGTTTAAAGTGGCTT11.0204081632653061No Hit
GATTGATTATCGCTCAAAGAAACATAGTAAGAAGAGCAGAAATGTCAGCA11.0204081632653061No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT11.0204081632653061No Hit
TCTGTTTCTTCTTTGTTGATTCATTGAAGGACTTCAGGTCAATGCTTGCT11.0204081632653061No Hit
GTATGGAAGCGTCACGCATAGGTGTAAATAGACAAACAAAGTCCCCATAC11.0204081632653061No Hit
GGCTAAGTACTGTTTGTGTTTCCATCTCCCAGCTGGGGAAACCGAGGCAC11.0204081632653061No Hit
CATTGCAGTTCATCTGTCCCGCAGGCTAAGTACTGTTAGTGTTTCCATCT11.0204081632653061No Hit
GGATGCGCGGAAATTAAGTATTGAAGCCCATCTTCAAGAGGCGGATGGAA11.0204081632653061No Hit
CGGTTGGTGGGGGTGGGGGGCGGGGGGGGGGGGGGGGCGGGGCTGGGGGG11.0204081632653061No Hit
GTATGCAAGAGTCCCGTTTTCGTTTAATTAAAAACACAACCTCACCATAC11.0204081632653061No Hit
ATATCCATAGGCTCTTAAGTATTCAACCCGGGGGAAATTGGGTGTGAGAC11.0204081632653061No Hit
GCACAAAGGGCTTGAATGAAAACCAGCTGCAGGGACAGAGAATCCTATTG11.0204081632653061No Hit
CCCCAGACAGGAGCCACACAAATGGGAAAAATACTGAGTTATTGAAATAG11.0204081632653061No Hit
GCAGGAATACCTGGGATCCTTTGGGCTGCTCCGGGGTGGCTGGTGGGTGG11.0204081632653061No Hit
GTCATACTCATCTGCATTGTCTCCGAAGAAATAATACCCTTCATTACTCA11.0204081632653061No Hit
TTGTAGAATCTGGTGACCCGAATGCACTATTGAAGCACCGATTTGAGATA11.0204081632653061No Hit
CTATTGTTCCAACTCCTTTCACCGCAGCACCTGCGGCACCAGACCTTATG11.0204081632653061No Hit
ATTATTGAAAGGGAAGTGCTGTCTCTGAACTTGGCAGGACTCAATAACTG11.0204081632653061No Hit
CACTTGTACAATATACGGAATCTTCACATTCATGAAGTGTGATTAAAATG11.0204081632653061No Hit
ATGCAGTTGCTGCCGGTTGATTTCTGTTTAATGTACAATCATAAGTCTGT11.0204081632653061No Hit
CCTGAGAGTACTGGCACCGTGAACTGTGATGAGGCCCAGAGGAGAAGGCA11.0204081632653061No Hit
CCTCCTCATGCTTGCGATATGTTCAATCGGGGCAACATCTTCTCCTATTT11.0204081632653061No Hit
CTACTCAAGATTGCGATATGTTCAATCGGGGCAACATATTCTACTATTTC11.0204081632653061No Hit
TCTCAGAGAACTCTTATTTCTTGATCCGTCCAGACTCGACGTCGACCCTG11.0204081632653061No Hit
ATTCATGTTGTACCCATCTTGCTATAGATCTGGGCTCTGTCTAATCAATG11.0204081632653061No Hit
CCGCTGCACTGAGAATGTAGGCTGCACACTGATATGGCCTGATGAAGCCT11.0204081632653061No Hit
AGAGAATAATGGACATGATTACAGAGAGTAATGAACAAGGACAAACAATC11.0204081632653061No Hit
CAGGAACAGGATACACCATGGACACAGTAAACAGAACACACCAATACTCA11.0204081632653061No Hit
CTAGATCCAGCCATCTGTTCCAAAGCGTTTGCCGAAGTGCTAGCCACCAC11.0204081632653061No Hit
CCCACGCACTTACTGTGAGTTGTTCCTCGACTGGGGTCCCTGAAAGTGTG11.0204081632653061No Hit
GGAAAGAACACAGATCTTGAGGCTCTAATGTAATGGCTAAAGAAAAGACC11.0204081632653061No Hit
GGTTGGGAGCGCGGGGTTTAGGGGGGGGGTGGGTGTCTGGGGCGGTGTGT11.0204081632653061No Hit
CCCTTATACTGGACATACTCCATACAGCCATGCAACAGGAACAGGATAAG11.0204081632653061No Hit
TGCTAGAGTCCCGGTTTACTTTCAATTACAACACAACGTCCCCATGCCCA11.0204081632653061No Hit
GACTGAATAAGAGAGGCTATCTAATAAGAGCACGGACATTAAATACGATG11.0204081632653061No Hit
AAGAAAACCCGCAGTAATACCTGTTATCATTTGCCATGCTCCGGGCTCCA11.0204081632653061No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGACA50.0145.01
TATGCAA50.0145.08
GTACAGG50.0145.0145
GACATAT50.0145.04
ACATATG50.0145.05
ATATGCA50.0145.07
ATGCAAG50.0145.09
AGACATA50.0145.03
CATATGC50.0145.06
TAGACAT50.0145.02
Produced by FastQC (version 0.11.5)