FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R1_C08-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R1_C08-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1130855
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3850.052.7828941
CGAAAGC5700.038.1237953
GCGAAAG6000.036.249682
ATATGCG2002.2737368E-1036.2079965
CAATTCG3000.033.7986159
CTATACG950.0047482730.4990393
TATGCGT2401.6025297E-930.1719976
ACTACCG1306.5258885E-427.8535378
GGTCGTT2652.2846507E-924.594117
TTAGTGC2502.3027424E-623.1731194
ATGCGTC3152.9012881E-822.9892047
GGATAGC3203.4284312E-822.635
TATACGA1600.002190030222.634
TATATGC4205.2750693E-1122.4144764
TGCGTCT3254.0385203E-822.282838
TATAGAC10800.022.1525822
TATACTG10850.022.0251435
GTATATA5450.021.3068561
ATAGACC6500.021.173373
CTATAAC2455.1500938E-520.6957763
Produced by FastQC (version 0.11.5)