FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R1_C06-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R1_C06-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1163874
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT12660.10877466117466324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2300.056.7954981
CAATTCG3500.047.6000259
GTAGAAA8050.041.4697271
CGAAAGC3550.036.75912
CCTTATA5200.036.2485582
TATACGT1406.657574E-736.2174074
GTATTAC8600.033.754431
AGTAGAA5850.033.4947781
CGACGTG900.003646025432.1904836
CTTAGAC7050.028.7758563
GTATATC1306.446055E-427.9123151
ATTGACG1306.5079617E-427.866733
GACAGTA4200.027.5942157
GCGAAAG4750.027.5009781
TTAGACA7600.026.686514
TAACGAC4350.026.6495633
ATTCGAC4400.026.3898281
ATAGACA9650.026.2784713
GATAGCA4700.026.1975776
TATTACA11150.026.0079362
Produced by FastQC (version 0.11.5)