FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R1_C05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R1_C05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTCAATTTTTCCGAAAAGACGCCAGATACCCCTATAGTGGCCATAGG18.333333333333332No Hit
TCTCAGTTCCTTGCGTTACACTGACTTCTTCGGGAGACAATAGTACGTTC18.333333333333332No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT18.333333333333332No Hit
CGTCTACTCAGTGCCTCTTGTCAACACATTCCTGATTAACTCCTTCGGTG18.333333333333332No Hit
GGAGTCTATGGGATTCCTTTCGTCAGTCCGAAAGAGGCGAAGAGACAATT18.333333333333332No Hit
GCCAGAGGGTTGAGGATTAGCTCATCGTTCAGTTTTGGTGGGTTCAATTT18.333333333333332No Hit
GTCTGTTACTCACCTTTATCATTGAAAGAGGGATCAAAACTCAAATTGGA18.333333333333332No Hit
CTTCGTTCCCTTTATCAGTAATGTAGGATTTGCTGAGGTTTGGGTATGAA18.333333333333332No Hit
AAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGGGCTA18.333333333333332No Hit
GGTATCACCTCTGGCCGTAACATGGTGGAAGAGGCATGGCCGAACCAGAA18.333333333333332No Hit
CTCAAGAATGGTTCAATTTTGGCGTTCACTTCTTTTGACATTTGGGAAAG18.333333333333332No Hit
GTCCATCCGTTCGGATACCACATCCGCTCAAAACCGGTTCTTTACCTAAT18.333333333333332No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)