FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R1_C04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R1_C04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318878
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAGAGAATTACTTAATATGTTAGTAGAGTGGATATTAGATGGTGGAG8840.27722200967141036No Hit
TGTTAGTAGAGTGGATATTAGATGGTGGAGACAATTAGTCGACCGCTATG8370.26248283042417475No Hit
CTATTATTGTTCCCATTTTACAGTTGAGAAAGCCAAGGCTCTGAGAAGCT7970.2499388480861019No Hit
AAGCAGGACCAATCAGAGAAGAGCCCGTCCTGCTTTGGTTCCCGCCCACA7820.24523485470932457No Hit
TCTTTATGCTCCCCAGAAAACCATTTACTAATGCCCATGCTTACCTCCTA7550.2367676666311254No Hit
ATATTTATCTATGGCTTCATTTTAGTGTCAAAACTCACACACGCGAGGAA7440.23331807148815534No Hit
GCTATTAAGGGTAGGCAGGAGAAGATGCATTTAAGACTTACTTAGGAGGT6850.2148156975394979No Hit
TCAGAGAAGAGCCCGTCCTGCTTTGGTTCCCGCCCACAGTGTCAAAAGTC6760.2119933015134315No Hit
GCATAAAGAAGGATATCAAAATAGGGGTGGCGAAGGAACTCGTGTTGCAA6440.2019581156429732No Hit
AAATTAGAGGATGTAAATTGATATTTATCTATGGCTTCATTTTAGTGTCA6400.20070371740916587No Hit
GTACTATTATTGTTCCCATTTTACAGTTGAGAAAGCCAAGGCTCTGAGAA6380.20007651829226225No Hit
ATCTATGGCTTCATTTTAGTGTCAAAACTCACACACGCGAGGAAGCGTGT5830.18282854257741205No Hit
GAGAAGAGCCCGTCCTGCTTTGGTTCCCGCCCACAGTGTCAAAAGTCATA5450.1709117593562428No Hit
CCCTGAAAGTGTGCCTTCTCCTCTGGGCCTCATCACAGTTCACGGTGCCA5450.1709117593562428No Hit
AATCAGAGAAGAGCCCGTCCTGCTTTGGTTCCCGCCCACAGTGTCAAAAG5330.16714856465482097No Hit
CTCCAGAGGCGCCCTACTCACTCGCTTTCAGGTTTGTCCAGCTAGCCAAA5300.1662077659794655No Hit
CCTCTGGGCCTCATCACAGTTCACGGTGCCAGTACTCTCAGGTGACTGGT5300.1662077659794655No Hit
ATATTAGATGGTGGAGACAATTAGTCGACCGCTATGGTAATCTGGCTAAA5230.16401256907030273No Hit
GGGTGGAGGTGGGATGGAGGGAAACATGGTGGGTGAGAAAAAGATTCGAT5210.1633853699533991No Hit
GTGTCAAAAGTCATAACTCGAGCGAGCAGATAAAGTTTTCGCGAGAGCGT5100.15993577481042906No Hit
AAATAGGGGTGGCGAAGGAACTCGTGTTGCAAGCCAACTCTGATTAACTG5080.1593085756935254No Hit
GGTTAGGACATCTGGTCTGTGGGTCTGGACTCCGAGTGCCAGGGTCTGAG4980.1561725801090072No Hit
CTATGAGCCCAGGCGGTGAGTACTATTATTGTTCCCATTTTACAGTTGAG4790.15021418849842258No Hit
ACTCTACTCTTCCCTGCCTCCCACTCCATTGCTTTGGTGTAGTTCACAAA4760.14927338982306712No Hit
CCCCAACCCCACGCACTTACTGTGAGTTGTTCCTCTACTGGTGTCCCTGA4720.14801899158925982No Hit
GTCCAGGAGGTCTGTGCTCCAGGAGGTCTGTGCTCCATCTTCACGGAGGA4660.1461373942385489No Hit
CACTTACTGTGAGTTGTTCCTCTACTGGTGTCCCTGAAAGTGTGCCTTCT4650.1458237946800971No Hit
GTGCCAGTACTCTCAGGTGACTGGTCGGAAATCAGGAGTCCCCCTCAGAC4650.1458237946800971No Hit
CCCCTATTTTGATATCCTTCTTTATGCTCCCCAGAAAACCATTTACTAAT4400.13798380571880153No Hit
GTCTAAAACCTGGTGCTAGGTCACAGAGCTTGTCACAAGAGAGGCAGGAT4360.13672940748499426No Hit
GTCATAACTCGAGCGAGCAGATAAAGTTTTCGCGAGAGCGTATGATAAAT4190.1313982149913133No Hit
CTTAATAGCTGTGCCCGAAATATGAAACTCTTGTTTTATTTTAGCCAGAT4170.13077101587440965No Hit
ATTGAGTGTTGACCGCGAGACAGAATGTCTGCAGAAGGTGGGAAGATTGC4140.1298302171990542No Hit
GGATATTAGATGGTGGAGACAATTAGTCGACCGCTATGGTAATCTGGCTA4140.1298302171990542No Hit
GCTTTGGTTCCCGCCCACAGTGTCAAAAGTCATAACTCGAGCGAGCAGAT4120.12920301808215054No Hit
CCGTAGAAGCTCCTGAAGAATGTCTTTCAGTCTCTGTCTTACTGGACCAC4110.12888941852369873No Hit
GAGGGAAACATGGTGGGTGAGAAAAAGATTCGATGAAAATTAGAGGATGT4100.1285758189652469No Hit
ATGGTAATCTGGCTAAAATAAAACAAGAGTTTCATATTTCGGGCACAGCT4100.1285758189652469No Hit
GCTTTGGTGTAGTTCACAAATCGTTTATGGTCCTTTTTCCCACTGAACTC4030.12638062205608414No Hit
GAAACATGGTGGGTGAGAAAAAGATTCGATGAAAATTAGAGGATGTAAAT4000.12543982338072868No Hit
CTCCATTGCTTTGGTGTAGTTCACAAATCGTTTATGGTCCTTTTTCCCAC3960.12418542514692138No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT3940.12355822603001775No Hit
GGCATGGAGTGTGGAGAACACGCAAGCATACTGGGACGATTGAGTGTTGA3900.12230382779621046No Hit
ACTCTCAGGTGACTGGTCGGAAATCAGGAGTCCCCCTCAGACAGAGGTGG3890.12199022823775865No Hit
GCTCTATGCTTCTGTTACTTGAGCAAACTTGTTGGGAGAGTGTTTTATGG3820.1197950313285959No Hit
GACATAATGTGAGTAAAGCCCTCATCACCTGCATGGCTGCTATGATAAGC3790.11885423265324044No Hit
GAATTACTTAATATGTTAGTAGAGTGGATATTAGATGGTGGAGACAATTA3770.11822703353633678No Hit
GATGTAAATTGATATTTATCTATGGCTTCATTTTAGTGTCAAAACTCACA3690.11571823706872221No Hit
ATCTGGAAGTCACGGTACAGAGAATTACTTAATATGTTAGTAGAGTGGAT3670.11509103795181855No Hit
CTATGGTAATCTGGCTAAAATAAAACAAGAGTTTCATATTTCGGGCACAG3650.11446383883491491No Hit
TATTTGGGGGGCGGTATGCGGAGATTTATTAAAATTTCAAATTTATCACG3600.11289584104265582No Hit
CTTGTTGGGTGGGTGGTTGGATGGGTGGATGGACGGATGGACAGACGCTT3590.112582241484204No Hit
CATCAGGAGGTTCCATCCAAAGTTCAAGGTTTCTCCTTCCATGAAATGTC3570.11195504236730036No Hit
CTCCAGAAACAACGCACTTCTTTCTCAGAGAGGTCTAAAACCTGGTGCTA3560.11164144280884852No Hit
CTACTAACATATTAAGTAATTCTCTGTACCGTGACTTCCAGATAGAACAA3520.11038704457504124No Hit
ATTTAAGACTTACTTAGGAGGTAAGCATGGGCATTAGTAAATGGTTTTCT3510.1100734450165894No Hit
CCCTCAGACAGAGGTGGAGGTGGCAAGCACGCGGCACCTACTCTACTCTT3510.1100734450165894No Hit
GTCCAAACACTTGTTGGGTGGGTGGTTGGATGGGTGGATGGACGGATGGA3450.10819184766587847No Hit
GTTTAAGGACCTTGGTGCTTCAGAATTACCAAAAGATTTTAGGACAGGAA3440.10787824810742667No Hit
GATAAAGTTTTCGCGAGAGCGTATGATAAATTCCAGCGAGCAGATCCAGT3380.10599665075671574No Hit
AGTAAATGGTTTTCTGGGGAGCATAAAGAAGGATATCAAAATAGGGGTGG3310.10380145384755299No Hit
GTCCTAACCAGGCCATAGGCCAAATGGGGCCTATGAACATATTTGGGGGG3260.10223345605529387No Hit
GTCAAAAGTCATAACTCGAGCGAGCAGATAAAGTTTTCGCGAGAGCGTAT3230.10129265737993841No Hit
GATGTGAGACGTAACTCTGAATTGTTCTATCTGGAAGTCACGGTACAGAG3220.1009790578214866No Hit
AAGTAGGAGGGCATGGAGTGTGGAGAACACGCAAGCATACTGGGACGATT3210.10066545826303477No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGAAA750.087.0088651
TCGAAAC851.8189894E-1276.6881942
CTTCCCG300.001931335372.53017145
CCGGAAT300.00194461572.405029
TAACGTT406.6996006E-572.405029
CGAAACC903.6379788E-1272.393673
TACCATG406.7048095E-572.393668
AACCATC300.001945825672.393665
ATAACGT406.7048095E-572.393668
GACCAAT1650.070.210937
GGACCAA1750.066.188496
AACCGTA554.08694E-665.822747
ATACCAT554.08694E-665.822747
AATAACG451.2003771E-464.3600167
GGTTGTT350.003558399962.16872145
TAATACC350.003585060662.0517125
GGCCAAG502.0071426E-458.005911
TGTATAG502.0180782E-457.94222
ACCAATC2100.055.1570788
CGAAAGC3450.054.557543
Produced by FastQC (version 0.11.5)