FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R1_C01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R1_C01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGTGAATAACAGAACTGCGAGGACTAATGTCGCAGTCCCGCACTCGCG12.127659574468085No Hit
CCCTAAGAGCCTCAACAATGAGAAGCAATTTTATAGAATCAGCCGAACAC12.127659574468085No Hit
GTTCCAGGTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTT12.127659574468085No Hit
CCATTACTCAGAAAAGGGAATTTGGAGGCCAACCCAAGAGACGGGTCCCC12.127659574468085No Hit
ATTCAATATGGAGAGAATAAAAGAAATGAGAGATCTAATGTCGCAGTCCC12.127659574468085No Hit
AAGTAATATATTTGGACTTCCATCCGTCTTACTCAAATTTCAATGAACAG12.127659574468085No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG12.127659574468085No Hit
CCCTCCAGCAATGGCACAGGAACCGGCTCCACCCGGGACAGGACACCGAC12.127659574468085No Hit
CACAAATCGTTTATGGTCCTTTTTCCCACTGAACTCGTTGTGGCCTCAGG12.127659574468085No Hit
GATCTAATGTCGCAGTCACGCACTCGCGAGATACTCACTAAGACCACTGT12.127659574468085No Hit
GATTCTCATTATTCAGGCACAAAGGCACAACCTCCATGCTGGAACCTTCG12.127659574468085No Hit
GTATTGGAGTCCATGGTTTCCACATTCTCATTTGAAGCAATCTGGACACC12.127659574468085No Hit
GTGATGAATAAAACTAACTCATGGCCATGCATCTCCGACATAAAAAATGA12.127659574468085No Hit
CAATCTGCACACATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTC12.127659574468085No Hit
GGATACAACATTATGCACTCTATTCGATAAAGTTTTCACTAGCAGTTCAT12.127659574468085No Hit
CTAGACACCATGTCCTCCACCATGAAAGATATTCAATACGTTCTCCGTAA12.127659574468085No Hit
GTAGAAACAAGGAATTTTTTAAAAAAATAGACAGAAATTGAAGCAGATCA12.127659574468085No Hit
AGATTGAATAGCCGGGATTTTCCGGATTCGTGTGGGAAGTTGCACGTTTG12.127659574468085No Hit
ATTACAAACAAGGCATATTTTCATGAATGACAAGTTAAATAAATTATTTT12.127659574468085No Hit
GAAGAAACAAGGTAGATTTTAATAAAAAAGAAACTAATTTATCTCCATAC12.127659574468085No Hit
ATTCTCTTGTCTGATGGAATTGGGTATCTAATTGCAATCATTCTGAGTGA12.127659574468085No Hit
ACCAAGGTCAGTGGATGAAGGCTATATTGGGAAAGTCTGCAGGAGCTAAC12.127659574468085No Hit
GTTAAAAAGAAGGGCTATCGCAACACCTGGGATGCAGATTAGAGGTTTCG12.127659574468085No Hit
GCACATAGTAGCATTGACACAACTACTTCAGAGGATCTGTGTGGAAGGAG12.127659574468085No Hit
GTGTTTTATGGTTCCTGCTTAGCTGCTTTGCTTTGCCACTTCAGGGACTC12.127659574468085No Hit
CCAAAATTAATGCCACACCAATTGAATGCTCGTATCTCCAGCTTACTATC12.127659574468085No Hit
GACTTTAAATAAAAAAGTTGAAGATGGTTTCCTGGCCTTTTGGACGTAGA12.127659574468085No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGATCTTA12.127659574468085No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12.127659574468085No Hit
GTATGGATTGCAAATAGTAGCATTGCCACAACTACTTCAGTGGTTGTGTC12.127659574468085No Hit
GAGGAGTGGTTAGGTTCACCCTCGGAAGGAGGAATGGCCTTGGCGAGGGT12.127659574468085No Hit
CTATGCTACCTTCCAGTGTTGGATTCCAGACGCCAACAAAGGCACCAACC12.127659574468085No Hit
GGTGCTAGCAGAGCTACAGGACATTGAAAATGAAGAGAAGATCCCAAGGA12.127659574468085No Hit
GCTGTTTGTAGTTTTCCTGTGTGTATATTGCAGAGTCTTTGCTTCGCTAC12.127659574468085No Hit
GCATTATGTAAAACAGTTGGCTAACATATACTGAACACCACTCTCGAGGC12.127659574468085No Hit
CAGCCAAACAATTAGAAAGGATGTCAATCCGACTCTAAAATTCAAAAAAA12.127659574468085No Hit
GTAGTGAAGGTCTCCCATTCTCATCACAGTTTCTCCACGCGAATCTCTGT12.127659574468085No Hit
CACCATGGGAGGATGAACAGATGTACCAGAAGTGCTGCAATCTATTCGAG12.127659574468085No Hit
GGGGCCGGGGCCAGCTGCATCTGCTGATGGTGGTGGTGATGGTGCTGCAG12.127659574468085No Hit
TACATGCAATCACCTTAAAAAGGTATATAAAGTAGGATGAATATATGTCA12.127659574468085No Hit
AAAAAAGTTCCATTTAACACATATCCACTATTAATTATCTCCATTAGAAA12.127659574468085No Hit
CTTCTTTCTTGAGCCGACCAGACTCGAAGTCGACCCAGCCATCAATCCGG12.127659574468085No Hit
GAGAGATGTAATGTCGCCGTCCCGCACTCGCGAGATACTCACTAAGAACA12.127659574468085No Hit
TAAAAGAACTGAGAGATCAAATGTCGCAGTCACGCACTCCCGAGATACTC12.127659574468085No Hit
GCATTAGCCATCTCTCCTTTTGCAAGATTGCACAGTTCATTGGCCCATAT12.127659574468085No Hit
CCTTTCACCGCAGCCCCTTCGGCACCAGACATTCTGGGAATGGTGGAACC12.127659574468085No Hit
GACTAGTTAATGGTTGCATGTTCTAGAGTAATAGAAGGAAGTTGCGCTCA12.127659574468085No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)