FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R1_C01-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R1_C01-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076506
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC2700.061.698143
CCGTACC606.849794E-660.3512659
GACCGTA606.851682E-660.348467
AGCGAAA4950.058.5986561
CTATACC2250.048.2765274
GCGAAAG6850.042.3213962
ACCGTAC1201.9912295E-742.2439238
GTATAGG1301.6524245E-533.4688451
GGGTGCG1102.4524803E-432.9173437
GTACGTA950.004752564230.4932699
TCGGATT1204.0541627E-430.213526145
ATATACT7950.030.0589714
TATTATA3201.8189894E-1229.4431252
CAGCTCG3700.029.3600759
TATACCA4850.028.3686815
TATACTT10250.028.2594325
TACAGGC1804.7128306E-628.1848742
TACCATG6250.027.808577
GAGCGAA2104.977519E-727.6250821
GTATTAC2903.947207E-1027.5060081
Produced by FastQC (version 0.11.5)