FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R1_B12-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R1_B12-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1247623
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG19960.1599842260041695No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT15030.12046908401015372No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT14770.11838512114637195No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT14760.11830496872853417No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG14670.11758359696799434No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC14030.11245384222637766No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT13870.11117140354097352No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG13010.10427829560692613No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA259.423713E-486.9152459
AGCGAAA2500.078.377671
ATCGCGT450.00967841348.284318
CAATTCG6050.044.2956479
CGAAAGC4650.043.6361273
CCTTATA4650.040.5534132
GCGAAAG5200.040.448432
GGTCGTT3400.036.210337
GTAGAAA12250.031.9908871
AGTAGAA8550.030.5565951
GGGTATA1000.006045667529.0287651
AGGTCGT4550.028.6499336
GGATAGC3550.028.5614075
CCTATGC2054.0565465E-728.2798583
TCGTTTT4750.027.4469189
TTTAAAC11100.027.4199963
CCCTTAT6900.027.3459381
TTATACT6400.027.1621044
ACAATTC10800.026.8246178
AACTATC2001.074833E-525.347237
Produced by FastQC (version 0.11.5)