FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R1_B05-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R1_B05-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1333595
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGAGCGGTGGCCACTTCCCTCAGAAACTGTCGATGCCTCTCAGAA19150.14359681912424685No Hit
GTCCTGGATGACCTCTTGGGAAGTTACCCAGCTTTCTTTCTGTGGTACAG18060.13542342315320618No Hit
ATATATATCTTTAGAGTCAAGAAAAGGCATCATTGCATTAGCAAAAAGGG17080.12807486530768336No Hit
GTATAAAGTCCCGCCCAAAACTGTACCACAGAAAGAAAGCTGGGTAACTT16870.12650017434078564No Hit
GGTTAAGAGAGTGGCCTCTTACTGTTTGCTTTTTGAGTCCTGCACATGTA14990.1124029409228439No Hit
GTTTTAGGATGGGGAGACCCAGACTCTGGGTGTCAGACTGAGGGAGGGAG14350.10760388273801268No Hit
GTGCAGGGACAGTCCGAGGTGCTTCCTGGGGAAAGAGAAAGGTGAACAAA13980.10482942722490711No Hit
GTACAGAGTCGGGGAGATCGGGGAGAACAGCGGGGTTTTCCAGAGCGGTC13570.10175503057524961No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA6350.050.2973251
CAATTCG1900.049.5520029
GTATGTA1850.047.0842321
GTATTAG2350.043.2440261
TATTAGG2350.043.2034342
ATTAGGA2350.043.1791153
ACATCGA1557.4760464E-1042.0515758
CGAAAGC9100.038.23083
GACATCG1604.6351488E-836.211087
TACTATA4100.035.3756332
ATCGGCG1352.1392338E-532.229923145
ATATATC9150.030.8927883
CTATCGA950.004752761730.493549
TCTACTG4800.030.1804265
TAACGGG2901.2732926E-1130.00717145
GGGCGCT2553.0577212E-928.4029756
GTATAAG5400.026.8845161
AGCGAGC1907.144492E-626.716911145
TAGTACA5450.026.5769399
CTGGGCG2808.265488E-925.8737874
Produced by FastQC (version 0.11.5)