FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R1_A07_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R1_A07_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3815
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA130.34076015727391873No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT90.235910878112713No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG90.235910878112713No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT80.20969855832241152No Hit
GTACTATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGAACTGAGAAGTT80.20969855832241152No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA80.20969855832241152No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC80.20969855832241152No Hit
GCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGTTTCTCCAAGCG70.1834862385321101No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA70.1834862385321101No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA70.1834862385321101No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA70.1834862385321101No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG60.15727391874180865No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT60.15727391874180865No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA60.15727391874180865No Hit
GTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTT60.15727391874180865No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG60.15727391874180865No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG60.15727391874180865No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT60.15727391874180865No Hit
CTCCAAAGCTGGGTAGCTCCATGCTAAAATTAGCCACAAATCCATAGCGA60.15727391874180865No Hit
GACTGAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATG60.15727391874180865No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC60.15727391874180865No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG60.15727391874180865No Hit
ATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGA60.15727391874180865No Hit
TTCCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTATCGGGGA60.15727391874180865No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA60.15727391874180865No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA60.15727391874180865No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG60.15727391874180865No Hit
CCTATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT60.15727391874180865No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT60.15727391874180865No Hit
TCCTATATGTGAGAACCAATGGAACCTCCAAGATCAAGATGAAATGGGGC50.1310615989515072No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT50.1310615989515072No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG50.1310615989515072No Hit
ATCAATAATTGTAGAATCTGGTGACCCGAATGCACTATTGAAGCACCGAT50.1310615989515072No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA50.1310615989515072No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG50.1310615989515072No Hit
GTGTTGAGAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGC50.1310615989515072No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA50.1310615989515072No Hit
GGCGTGGTGTCGTCCTCAAGAATGGTTCAATTTTGGCGTTCACTTCTTTT50.1310615989515072No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC50.1310615989515072No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG50.1310615989515072No Hit
GTATGACAGTGATGAGCCAGAGCCCAGATCTCTAGCAAGCTGGGTCCAAA50.1310615989515072No Hit
GTATACGAAACCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTT40.10484927916120576No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC40.10484927916120576No Hit
GACCTGAAGTACTTCAATGAATCAACAAAGAAGAAAATTGAGAAAATAAG40.10484927916120576No Hit
GGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGA40.10484927916120576No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC40.10484927916120576No Hit
AGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGGACATACTT40.10484927916120576No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG40.10484927916120576No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT40.10484927916120576No Hit
GACAAGAAATGGCCAGTAGGAGTCTATGGGATTCCTTTCGTCAGTCCGAA40.10484927916120576No Hit
AAATGAATCAGCTGACATGAGTATTGGAGTAACAGTGATAAAGAACAACA40.10484927916120576No Hit
ACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCAT40.10484927916120576No Hit
CTATGGTGTTGGCCAATGCAGTTGCTGCCGGTTGATTTCTGTTTAATGTC40.10484927916120576No Hit
CTTCATACCCTTCATGTACTCTTATTTTCAGTGTTTGGAGGTTGCCCGTT40.10484927916120576No Hit
CTATAAGACAAGAAATGGCCAGTAGGAGTCTATGGGATTCCTTTCGTCAG40.10484927916120576No Hit
CTGGAGAATTGCCTCTTACCAGTATCCTCAACCCTGATCCTCTCACATTC40.10484927916120576No Hit
GTGTTTATATTGAAGTGTTGCACTTAACCCAAGGGACGTGCTGGGAGCAG40.10484927916120576No Hit
GTAGGAGTCTATGGGATTCCTTTCGTCAGTCCGAAAGAGGCGAAGAGACA40.10484927916120576No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA40.10484927916120576No Hit
GTCTTACTCATATCATGATTTGGCATTCCAACCTGAATGATGCCACATAT40.10484927916120576No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA40.10484927916120576No Hit
CCCTTGTCCTTCGTCCATTTTCACCCCTCCAGAAATTTCGGTCATTGATT40.10484927916120576No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG40.10484927916120576No Hit
ATACTGATGTGGTGAACTTTGTAAGTATGGAGTTCTCACTCACTGACCCG40.10484927916120576No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG40.10484927916120576No Hit
CTCTTGAGGACTGCGATAGGCCAAGTGTCGAGGCCCATGTTCCTATATGT40.10484927916120576No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG40.10484927916120576No Hit
GCACCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACAT40.10484927916120576No Hit
CCATAGAAGTCTTTAGATCGAATGGCCTAACAGCTAATGAGTCAGGAAGG40.10484927916120576No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC40.10484927916120576No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC40.10484927916120576No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT40.10484927916120576No Hit
GGATAAGAGTCAGCAAAATGGGAGTAGATGAATACTCCAGCACGGAGAGA40.10484927916120576No Hit
CCCTTCATGTACTCTTATTTTCAGTGTTTGGAGGTTGCCCGTTAGCACTT40.10484927916120576No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG40.10484927916120576No Hit
GAACATGAAGAGAACAAGCCAATTGAAGTGGGCACTCGGTGAAAATATGG40.10484927916120576No Hit
CAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATGTTGTGAGAAAG40.10484927916120576No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT40.10484927916120576No Hit
GAACTGAGAAGTTGACAATAACTTATTCGTCATCAATGATGTGGGAGATC40.10484927916120576No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT40.10484927916120576No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCCTG100.0068172025145.05
Produced by FastQC (version 0.11.5)