FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R1_A03-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R1_A03-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1121128
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2300.044.164661
CCTTATA3850.043.316252
CGAAAGC2750.036.8866273
AGGTCGT3550.032.650346
GGTCGTT3300.030.733377
CAATTCG3850.030.1048159
GTAGAAA11500.029.6534161
AGTAGAA10700.027.801921
TAGAAAC12350.026.9876143
TATACAT8400.026.7360924
TTATAGG4350.026.6873471
TATATTC6300.026.4521473
TCTACAC5600.024.5832423
CTATAGG2401.5876176E-624.185411
CCTAATA2101.5459753E-524.185411
GCGAAAG4201.8189894E-1224.1692122
GACCGTT1500.00150164224.1465728
GTCATAC4251.8189894E-1223.9008751
ATGCGTC1550.001816926623.3686947
TACATAC7750.023.3686926
Produced by FastQC (version 0.11.5)