FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R1_A02-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R1_A02-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences996238
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2400.096.728421
CGGTATC502.317478E-672.4406743
GCGAAAG3400.068.223992
CGCTCCG400.00604762554.38239145
CGAAAGC5050.045.9033
TGTTCGG1201.9713298E-742.297413145
GTAGGTA3000.041.037358
GTAACGG951.0356949E-438.1151859
TATAGAC3700.037.2235642
TAAAGTA3600.036.203974
CCAGTAC2154.9112714E-1033.6933333
TATACGT1751.0106487E-733.137372
TATATCC5500.031.5961954
ATATATC5050.031.5583113
CGTGCGT950.004753252530.4921478
TCTAGCG1204.061973E-430.2033272
CTAGCGT1255.1785784E-428.9762693
TGTAATA5350.028.4532322
TCAATAC1555.531167E-528.041553
TAGGTAA4400.027.9800119
Produced by FastQC (version 0.11.5)