FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R1_A01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R1_A01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTGACACAGAACCCATCCACCCAGGGGGAGTTCAAACTAAGGGCACTC12.7777777777777777No Hit
TCTTTGATTCCTCCTCATCACCCAGAGTGAAGCCATTAGGAAATGCTGCA12.7777777777777777No Hit
GATAACTTATAATTTAAGTGAGCAGATGGCAAACCTGAAGAAGCACTTGC12.7777777777777777No Hit
GCATTATTTTCTCAATTTTCTTCGTTGTTGATTCATTGAAGTACTTCAGG12.7777777777777777No Hit
ACTTACTAGTTTCTGTTCCATGCTAGCCTGTGCAACTCGCACTACAAGAT12.7777777777777777No Hit
TAGTAAGTCCGAAAGAGGCGAATAGACAATTGAAGAAAAATTTGAGATTA12.7777777777777777No Hit
CATCATGAATTGTCAAAGTATGAACAGCGCATCATAAGACATTAGTTCAC12.7777777777777777No Hit
CCATATGGCCAAAATAAAAAAGTACACAAAAGGAAGGCAAGAGAAGAACC12.7777777777777777No Hit
ATAATAATCATCCATTATATCACATTTTAAGAAGCCTTCAGGAATGTGAA12.7777777777777777No Hit
GTATCTCTTTTATTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGCAG12.7777777777777777No Hit
ATATGGGGAAGATCCTAAAATCGAAACTAACAAGTTTGCTGCAATATGCA12.7777777777777777No Hit
GATTTGTGGCTAATTTTAGCATGGAGATAACCATCTTTGGAGTGTCTGGA12.7777777777777777No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC12.7777777777777777No Hit
AGTCAATAAAAGAATATTGCGAAGATCCAAAAATCGAAACTAACAAGTAT12.7777777777777777No Hit
TGCCAGTTGCATGGGCCTCATATACAACAGGATGGGAACAGTGACCACAG12.7777777777777777No Hit
GTTAGATTAGCGAATAGTAGAATATCCACACCGAGATCTCTGCTTGAGTG12.7777777777777777No Hit
CATTTGGAAGTTTGTTTAATGTATTCGGATTACCATTTCATCGACGAACG12.7777777777777777No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG12.7777777777777777No Hit
ATTCGAGACCATCCCACCAGAAAATTTTCTTGTACAATTTCAATAGTCCA12.7777777777777777No Hit
ACTTTGTGCGACAATGATTCAATCCAATGATAGTCGAGCTTGCGGAAAAG12.7777777777777777No Hit
ATATACCTAAAGGTAATTAAATGTACTTGTTCTTCAGCCATTAATATTAC12.7777777777777777No Hit
GACGAAAGGAATCCCATAGACTCCTACTGGCCATTTCTTGTCTTATAGTG12.7777777777777777No Hit
ATTCTGAGCAATATATTATAAGAGTCCATAATTAAACAATTTTCGTCATA12.7777777777777777No Hit
AACTTATACAAGATACGCAATCTTCACATTCATGAAGTCTGCATAATAAC12.7777777777777777No Hit
TACCAGTTCCTGCTTATCCAGTGATAAGTGTTTAATAGAACTTACTCAGT12.7777777777777777No Hit
GTATGGTAGAGTCCCCTTTTAGTTGGAATTGAAACAGTTAGAGCCCATAG12.7777777777777777No Hit
AAGACAACTGTGTACAATATGTCAAAAATCAAAAAGTACTGTCTCTAATA12.7777777777777777No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA12.7777777777777777No Hit
GTCCTGCAATGATGATGCCCATATTACACAGTCTAAGTACTGTGTTCCTA12.7777777777777777No Hit
GATCTGAATCATCTAAGAGAAGTCGACTTAATTTCTCCCTAGACACAATT12.7777777777777777No Hit
TACCGAATGAAGTGATTGTACATAACGACGGCCTTTAGCCATGCAGAATG12.7777777777777777No Hit
GTCCTTGTTCATTCCTCTCTGGAATAATGTCCATTATTCTATTGTCTGCT12.7777777777777777No Hit
GATAAGTGTTGACTAGCACTGACTCAGGTACATTGATCTCCCACATCATT12.7777777777777777No Hit
GCCAAAGAATGTCTTCATGCATTAGATTGCATCATATAATGGTATTACCT12.7777777777777777No Hit
ACTCCATGAACAAAAATCACTTGTCTGATATAAAAACATAACACCAGTCT12.7777777777777777No Hit
CGATAAAACCATTTGAAAAAAAGACGATCAGTAATCCACAATATCCGGTG12.7777777777777777No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)