FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_H12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_H12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGTAGAGGGGTTGCGTGTGGGGGGCGAGGGGAGACGGAGCGGCCGACC15.88235294117647No Hit
ATACAATTCCTAAAAATAAAAAAGAAAAATCAAATAATCACAACATTAAA15.88235294117647No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA15.88235294117647No Hit
CAGGCAAACCATTTGAATGGATGTAAATCATACTCTACTTTTCCTAAAAA15.88235294117647No Hit
ATCCACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTATTCTCTT15.88235294117647No Hit
GTATGGATAGCCAATAGTAGCATTGCCACACCTACGTCAGTGCATGTGTG15.88235294117647No Hit
ACTCGCACATTAACATTCAATGAGGACAATTGAATCCGACTCGGGGCGGG15.88235294117647No Hit
TGTATACTTATAAGAGTGTAAATATATAATAGATTTCAAGCAATATGATA15.88235294117647No Hit
GGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGG15.88235294117647No Hit
CATCAATCCGGGCCCTAGACACCATGGCCTCCACCATGCTTGAAATTCCA15.88235294117647No Hit
CACCAATTCTCAGAAAAGGGAAAGTCGCCTACAAACACAGAGACTGGATA15.88235294117647No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGATGATCCGAAAATCGAAACTAA15.88235294117647No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGGTTTGGGTTCATTAGC15.88235294117647No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA15.88235294117647No Hit
GTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAAACCCCTCA15.88235294117647No Hit
AGTGATAATTAATCAGGCACTCCTCGATTGCTTCATAGAGCCCCCCAAGA15.88235294117647No Hit
CTCTTCTTTCTTCTCTTTTGTTATTGCCAGCTGTGACTCTGATGTCAGTA15.88235294117647No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)