FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_H07-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_H07-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1063427
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACTAAGAGTATCCCCAAGTCGCACTGAAAGTTTCTTACCAAAAGCTTT10750.1010882740423179No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCGC1051.6370905E-1162.088629
AGCGAAA5700.047.0778921
CGAAAGC7750.034.5875743
TACTCCG4700.032.3622975
CAATCGG1851.6407648E-731.357908145
GCGAAAG8600.031.1881052
TAGGCTT4150.029.6700845
TATACAG5150.028.1281195
TACACCG1050.007745136527.5923465
CTCCGTA5400.026.8284157
CAGTCCG5200.025.074259
ACTCCGT6500.024.515896
GACCGTT2451.92685E-623.6528058
TAGGCCC3402.5320332E-923.4343074
GTATTAA6200.023.395291
AGAGTCG3452.9958755E-923.0957668
CATAGAC2552.724617E-622.7284553
CTACTCC6800.022.3691124
GTATATA11700.022.3155081
CAAACGT4607.2759576E-1222.0459589
Produced by FastQC (version 0.11.5)