FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_H05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_H05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTTTCTCTCACTGACCCAAGACTTGAGCCACACAAATGGGAGAAGTA13.571428571428571No Hit
CAACAGGATGGGGGCTGTGACCACCGAATCGGCATTTGGCCTAATATGCG13.571428571428571No Hit
ACTGTTTATAGCTCCATGGGGTGTTTGACACTTAGCGTCAAATTCATCAA13.571428571428571No Hit
CTATTGTAAAAGTGTGCAGGACCTTAATGGCAAAATCAGTAAAAAACAGT13.571428571428571No Hit
ATATAGGACAGCTCATTACGGCACTTTAAGGGCTTCTGTCCTTCACGGAT13.571428571428571No Hit
GTGTTCGAATCTTCATTCTTTTACTCTAGAAAATGTACACTTTCCCTAGT13.571428571428571No Hit
GCATTGAGGATCCAAGTCATGAAGGAGAGGGAATACCACTATATGATGCG13.571428571428571No Hit
CCTCCATACAGCCATGGACCAGGAACAGGATAAACCATGGACACAGTAAA13.571428571428571No Hit
AAAAAATGTCGTTTTTAAACAATTCGAAAATAATTGATGGCCATCAGAAT13.571428571428571No Hit
CAGATAAAAAATCATATGGAAAATTTTGTACAAAATAAATAACAACTTCA13.571428571428571No Hit
GCATGAACAAGTCCATGGACAGTTATTCAATAGTCATTTCAGTTAGTTAT13.571428571428571No Hit
AGTCAAGAGAGAGCAAGAAGTGCTTACGGGCAATCTGCAAACATTGAAAT13.571428571428571No Hit
GTCCATATATTTGGATCCCCATTCCCATTAAGGGCATTTTGGACAAAGCG13.571428571428571No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT13.571428571428571No Hit
TTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAGCTATCTCA13.571428571428571No Hit
AACTACGTCAACTTGCCAAATTAGCCAAGTAGCCTTATCTCATATTTCAA13.571428571428571No Hit
ACCCAGAGGTGGTGGTACAGGTGGGAATAGTGACTCGGATAACTTGGACA13.571428571428571No Hit
CACTACGTCCCCTTGCGCATATAGCACCTTAGCCTTCTCTCATTTTGCAA13.571428571428571No Hit
CGTTCTATGATTCGTAGCAAAAAAGCAAAAAAAATGCCATTAACGCGATT13.571428571428571No Hit
ACCACCAATCCACTAATCAGGCATGAAAACAGAATGGTGCTGGCTAGCAC13.571428571428571No Hit
CAATATGTATAGGCTACCAAGCGAACAACTCAACCGACACAGGTGACACA13.571428571428571No Hit
GGCATGCGCTGCTCTGGTCGGCATGTGTGTAGTCGGGGGTGGGGGGCAGC13.571428571428571No Hit
GAACACACCAATACTCAGAAAAGGGAAAGTGGAAGACAAACACAGAGACT13.571428571428571No Hit
GCCATTAGAGGCCAATACAGCGGGTTTGTTAGAACTCTGTTCCAACAAAT13.571428571428571No Hit
CCATGGAGGTTGCTAGCCAGGCCAGGCAGATGGTGCAGGCAATGAGAGCC13.571428571428571No Hit
CTGCTAGCCATTTCTTGTCTTATGGTGAATAGTCTGGTTTTAATCCTACC13.571428571428571No Hit
CACTATGTCAGTCATAAACAGATTTATTCTGATAACAATCCTGAGCTAAT13.571428571428571No Hit
CAATCAGACTCTCTATTTGTTGAAGTGATTGGCGGCGGCAACGGCTCATC13.571428571428571No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)