FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_H04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_H04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGCAGGCAGGATTTATGTGCAACTGATCCCCTCAGAATGAGTGCTG11.282051282051282No Hit
GCCCAGGGGGGGTGCAGTGTGCTTCGCTGGAGAGTGTGGGCAGCTGGGTC11.282051282051282No Hit
ACCAGGGACACAGGAACCAGCTCACACCAATACTCAGAACAGGGAAAGTG11.282051282051282No Hit
CATTTGAGTTAAAGAAGCTGGGGGATCAAACCCAATCAAAGGTAGGGCTA11.282051282051282No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG11.282051282051282No Hit
CCTTCCGTAGAAGGCCCTCTTTTCAAACCGTATTTAAAGCGACGATAAAT11.282051282051282No Hit
CTACAGGACATTGAAAATGAAGAGAAGATCCCAAGGACAAAGAACATGAA11.282051282051282No Hit
TTCCAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGA11.282051282051282No Hit
GCTTTAGGCTGGGCCGGCGAGGCAGATAGTGGGCGGTATGGACGCGGTGT11.282051282051282No Hit
AAGTCATCCATGGCTGCACAGGATGCATTGAGCAAGGCCGTATTTATGTA11.282051282051282No Hit
TCCATCCACATAGGCTCTAAAATTCTCAAGGCAGGAGAAGTTCGGCGGGA11.282051282051282No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA11.282051282051282No Hit
GCGGGGGGGGGCAGGGTCTGCCGGGCCGGCAGGTGTGGGGGAGTCGGCGG11.282051282051282No Hit
CCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT11.282051282051282No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG11.282051282051282No Hit
GAAGTCGGGAGGATGACCCGAGGTACAAGAAGGGATGCAATCTCTGCGAG11.282051282051282No Hit
GACTGAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATG11.282051282051282No Hit
GTTTGCGGGCTGGGTGCAGGGGGGGCGGGGCGCCCGACCCCGGTAGGGCC11.282051282051282No Hit
AGTTAGTGGGAATCAACATGAGCAAAAAGAAGTCCTATATAAATAAGACA11.282051282051282No Hit
GAGATTTCTTTCACAATCACTGGGGACAACACTAAGTGGAATGAAAATCA11.282051282051282No Hit
CTGCTCCTGCGGGCAATGTGTTCAATTGGAGCTACATCTTCTCCAATCTC11.282051282051282No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.282051282051282No Hit
CGCAGAATGCATTAAGCATGGATGAGGTGAGCGATCTGGCGTATGTGCGA11.282051282051282No Hit
GCCGTAGACATATGCAAGGCAGCAATAGGGTTGAGGATTAGCTCATCTTT11.282051282051282No Hit
CTCAGAATGAAGTGGATGAAGGCAATGAGATACCCAATTACAGCAGACAA11.282051282051282No Hit
GTATTGCTCCAATAATGTTCTCAAACAAAATGGCGAGACTAGGTAAGGGG11.282051282051282No Hit
CAATTATTGCCTCAGCAATTGACTGCTCGTCTCTCCCGCTTACTATCAAC11.282051282051282No Hit
CGACTAGGGTATGAAGGACGCGTGAACTGAGCAGCAGGTCCTGGGCCAGT11.282051282051282No Hit
AAGCTGTTCTGGATCAGTCGTCCCTCATAATCATTGAGTTTAAGCTCGGT11.282051282051282No Hit
CATCATTGATGACGAATAAGTTATTGTCAACTTCTCAGTTCCTTGCGTTT11.282051282051282No Hit
GGGTTTGTATCAACTGTCCTCCGTATTTTAACTTGATTTCTGAAGTGGAC11.282051282051282No Hit
NTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA11.282051282051282No Hit
CTTCACATTCCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTA11.282051282051282No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGGTTTCG11.282051282051282No Hit
GCTTTGGGTATGAATTTCCTTTTTTAACTAGCCATATTAAATTTTTGTAG11.282051282051282No Hit
GAGGGAATACAAGCAGGAGTGGACAGATTCTCCAGGACCTGCAAGTTAGT11.282051282051282No Hit
ATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGA11.282051282051282No Hit
CGTCTTGGCTGGCGGCCTGCTTGTGGGTATGGGCTTGGGGGGGCTAGTGG11.282051282051282No Hit
GCTTATGAGAGAATGTGCAACATTCTCAAAGGGAAATTTCAAACAGCTGC11.282051282051282No Hit
CCCATAGACTCCTACTGGCAATTTCTTGTCTTATAGTGAAAAGACTAGTT11.282051282051282No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC11.282051282051282No Hit
GTGCATGAGGGATATGAAGAGTTCACAATGGTTGGGAGAAGAGCAACAGC11.282051282051282No Hit
GATTAAAACCAGACTATTCACCATAAGACAAGAAATGGCTAGCAGAGGCC11.282051282051282No Hit
GTACTATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGAACTGAGAAGTT11.282051282051282No Hit
ACGTAGCTCCCCAGCCCTCGCCCCGAGGGGTTCAGGGGGGGGCGGCGGCT11.282051282051282No Hit
GTATACGAAACCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTT11.282051282051282No Hit
GCACGGAGATGTCGCTGAGTGGCAGAAGCGTCAGCATAATGGGAGTAGAT11.282051282051282No Hit
CCTGTAGCTCTGCTAGCACCTGCTTCCAAGCCATGAGGTAATTGGGATTT11.282051282051282No Hit
AGTTATTGTCAACTTCTCAGTTCCTTGCGTTTCACTGACTTCTTCGGGAG11.282051282051282No Hit
GTTGCATGGGCCTCATATACAACAGGATGGGAACAGTGACCACAGAAGCT11.282051282051282No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT11.282051282051282No Hit
CTGGTGGGGAAATTAGACATGTGTTTACTTGAGCATCACCAGCTTTGCTG11.282051282051282No Hit
ATGCTGTGGGGGGTGGGGCCTGTCGGGGGGCGGTGGTCGGGGGTGGGCGG11.282051282051282No Hit
GCTAATAACGTCCTGCATACTTTCCCAGTGGAGCCTTCTTCCACTCCTGT11.282051282051282No Hit
GAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCAGCAATAGGGT11.282051282051282No Hit
AGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACT11.282051282051282No Hit
GTCAAGAAAGAAGAAGAAGTGCTAACGGGCAACCTCCAACCACTGAAAAT11.282051282051282No Hit
CTCAAAGCCGCGATAGCGCAGAGACTTGAAATTGTCGGTGCAGGAAAGCA11.282051282051282No Hit
GGAATGGAGATCAGGCGTTTCCTCCTCCGATCACTTCAACAAATAGAGAG11.282051282051282No Hit
CTTCCAAGCCAAGAGGTAATTGGGATTTATGCCTTTCTCATGTGGTTTGA11.282051282051282No Hit
CATTGAAGTACTTCAGGTCAATGCTTGCTAGCATTTCTGCTGGTATTTGT11.282051282051282No Hit
AGTTAAGAGGTATTGGCGTGGCAGAACACGGCTCTTGATGCGCGCATTGA11.282051282051282No Hit
GCGACTGAACCCCATGCACCAACTCTTGAGGCATTTCCAAAAAGATGCAA11.282051282051282No Hit
ATTCAAGCTGGATAGAGCTGGATGTTATAGGAGATCATGATGCGCCGATT11.282051282051282No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG11.282051282051282No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA11.282051282051282No Hit
CACTAGAACTATGACTTCGTGACCTGTATCCTTAAGTGAGTATTGTATGA11.282051282051282No Hit
CAGCAGAAGTGTCCCACTGCAGGGCTACTGAATACATAATGAAGGGAGTG11.282051282051282No Hit
GGGTGGGGGCTTGCGGGGGGGGGGGGGGGGGACGGTGGATGGGCCGGGGG11.282051282051282No Hit
GGTTAGCCGCATTCAAGTTACTCCAACAGCGCCAAGTGGTCCGCGTGATG11.282051282051282No Hit
CAGAATGGTTCTGGCAAGCACTACAGCTAAGGCTATGGAGCACGTGGCTG11.282051282051282No Hit
GTCCCTTGACATTTCTTCAATAGTCATGTCAGTTAGGTATCGAGAAGCAG11.282051282051282No Hit
ATCCAATGATCGTCGAGCTTGCGGGAAAGGCAATGAAAGAATATGGGGAA11.282051282051282No Hit
CACTAGAATCGCTCAATCTTCACTACCATTCGCTTCTGGTAGCCCTGAAA11.282051282051282No Hit
GTGCATTCACTATGAGAGCAAAATCGTCGGATGATTGGAGCCCATCCCAC11.282051282051282No Hit
CATTATAAATGAAATTGTAACGCAAAGCTCAGAAAATCATACTGAAAGGA11.282051282051282No Hit
CATTATAAAAGGAAGGTCTCATTTGAGAAATGATACTGATGTGGTGAACT11.282051282051282No Hit
ACATAGGCTCTAAAGTTTTCAAGGCTGGGGAAGTTCGGTGGGAGACTTTG11.282051282051282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTCT50.0145.06
GTTCTGG50.0145.08
ATGGTTC50.0145.05
TTCTGGC50.0145.09
GGTTCTG50.0145.07
GAATGGT50.0145.03
AGAATGG50.0145.02
AATGGTT50.0145.04
CAGAATG50.0145.01
CAGCGCT50.0145.0145
Produced by FastQC (version 0.11.5)