FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_H04-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_H04-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1013608
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG31200.30781130377818644No Hit
ATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGA26560.26203423808809717No Hit
CTTAATATGAGTGCAGACCGTGCCAGAAAGGTGAGATCTTCGATCTCAGC25500.25157654635717164No Hit
TGTCAAAGGAGGGCACGATCGGGTTCGCTGCCCTTTCGTCCGAGAGCTCG22500.22197930560926907No Hit
CTCATAAGCAATCCTTGTTTTTCTTCCATTCTCACCCCTCCAGAAGTTTC20850.20570082319792266No Hit
CATTATGATCTGGCATTCCAATTTGAATGATACAACTTACCAGAGGACAA19640.19376326942960195No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA19550.1928753522071649No Hit
CATATTAAGAGGGTCAGTTGCTCACAAGTCCTGCCTGCCTGCCTGTGTGT18870.186166644304307No Hit
CCCATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTA18460.1821216880687603No Hit
GCTTATGAGAGAATGTGCAACATTCTCAAAGGGAAATTTCAAACAGCTGC17830.17590626751170077No Hit
CTATGGTATCAAGTACTCTTGAACTGAGAAGCAGGTACTGGGCCATAAGG16080.15864121040875762No Hit
TCATTACTCATGTCAAAGGAGGGCACGATCGGGTTCGCTGCCCTTTCGTC15760.15548417139564802No Hit
ATACCATAGTATCCATGTTTTCATTTGAAGCAATTTGTACTCCTCTAGTG14520.14325064521984832No Hit
GCATAAATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC14040.1385150867001839No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCTGCTGT13670.13486476034127592No Hit
CCCTAGGAGATCTGGAGCTGCTGGCGCTGCAGTCAAAGGAGTTGGAACAA12960.127860080030939No Hit
CATTAATACTGCTTTGCTTAATGCATCCTGTGCAGCAATGGATGATTTCC12650.12480169848698905No Hit
GATCTATGCTGGGAGTCAGCAATCTGTTCACAGGTTGCGCATATTAGGCC11980.11819164805329083No Hit
ATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGACA11960.11799433311497148No Hit
CTCTAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCT11660.11503460904018122No Hit
GTGCAGACCGTGCCAGAAAGGTGAGATCTTCGATCTCAGCATTTCCTGGG11620.11463997916354252No Hit
GTGTATGGACCTGCCGTAGCCAGTGGGTACGACTTCGAAAAAGAGGGATA11450.11296280218782803No Hit
ATCCAGAACAGCTTAACAATAGAGAGAATGGTGCTCTCTGCTTTTGACGA10630.10487288971673468No Hit
GCATAGATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC10320.10181450817278474No Hit
CTCCAGAAGTTTCTGTCATTGATTCCACGTTTGATCATCCTGATCAACTC10310.10171585070362507No Hit
CGTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCGATCAAGAATCCA10290.10151853576530571No Hit
GGATAGACCCTTTCAAACTGCTTCAAGCCAGCCAAGTGTACAGCCTAATC10220.100827933481188No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGGG203.8645102E-4108.76104145
TAGGGGC203.8645102E-4108.76104145
TCGGTCC259.382999E-487.00883145
AGCGAAA850.085.34071
GTAGAAA6100.071.3504261
TAGATAT2250.070.85063
GGGTAGA1550.070.1996151
CCATAGG955.456968E-1268.7217251
TAAGCCT350.003565083362.149162145
GTACTGA1200.060.3721166
GATAGCA3100.056.087646
AGGGTGT3150.050.5975846
GGTCGTT1152.5011104E-950.397597
CAAGGGT6650.050.1133964
AAGGGTG3250.049.0407335
TTAGTAG604.931812E-448.3597341
ATGCGCC450.00963530748.338238145
TGGGCCC450.00963530748.338238145
GGATAGC3450.048.2976955
ACAAGGG7250.047.9740753
Produced by FastQC (version 0.11.5)