FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_H03-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_H03-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1180200
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGA25550.21648873072360617No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCTGCTGT24880.2108117268259617No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG24470.2073377393662091No Hit
CTTAATATGAGTGCAGACCGTGCCAGAAAGGTGAGATCTTCGATCTCAGC24180.20488052872394508No Hit
ATATACAACAGGATGGGGGCTGTGACCACCGAATCGGCATTTGGCCTAAT21890.18547703779020505No Hit
CTCTAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCT21740.18420606676834433No Hit
CGTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCGATCAAGAATCCA21280.18030842230130487No Hit
GCATAGATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC20960.1775970174546687No Hit
CTCATAAGCAATCCTTGTTTTTCTTCCATTCTCACCCCTCCAGAAGTTTC19880.16844602609727166No Hit
CTCATAGACTCTGGTACTCCTTCCGTAGAAGGCCCTCTTTTCAGACCGCG19740.16725978647686834No Hit
GATCTATGCTGGGAGTCAGCAATCTGTTCACAGGTTGCGCATATTAGGCC19530.16548042704626334No Hit
CATTATGATCTGGCATTCCAATTTGAATGATACAACTTACCAGAGGACAA18490.1566683612946958No Hit
CTTTTCAGACCGCGTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCG18490.1566683612946958No Hit
TGTCAAAGGAGGGCACGATCGGGTTCGCTGCCCTTTCGTCCGAGAGCTCG17790.1507371631926792No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA17580.14895780376207424No Hit
CATATTAAGAGGGTCAGTTGCTCACAAGTCCTGCCTGCCTGCCTGTGTGT17260.14624639891543806No Hit
CCCATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTA16760.14200982884256905No Hit
GCTTATGAGAGAATGTGCAACATTCTCAAAGGGAAATTTCAAACAGCTGC16520.13997627520759193No Hit
AATATGGACAGAGCCGTTAAACTGTATAGAAAGCTTAAGAGGGAGATAAC16420.13912896119301815No Hit
GCATAAATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC15470.13107947805456704No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA15460.13099474665310964No Hit
GTATAGAAAGCTTAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATAG15430.1307405524487375No Hit
CATTAATACTGCTTTGCTTAATGCATCCTGTGCAGCAATGGATGATTTCC14810.12548720555837992No Hit
GTTTTTTACTCTAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCAC14040.11896288764616167No Hit
CCCTAGCTCCAGCGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG13850.11735299101847145No Hit
TCCCAACACTGTGTCAAGCTTTCAGGTAGATTGCTTCCTTTGGCATGTCC13540.11472631757329266No Hit
CTGGTACTCCTTCCGTAGAAGGCCCTCTTTTCAGACCGCGTTTAAGGAGA13460.11404846636163361No Hit
CAACAAGAGGATCACTTGAATCGTTGCATCTGCACCCCCATTCGTTTCTG13280.11252330113540078No Hit
CTATGGTATCAAGTACTCTTGAACTGAGAAGCAGGTACTGGGCCATAAGG13270.1124385697339434No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAGCTATCTCAATGCATGTGTT13210.11193018132519912No Hit
ATACCATAGTATCCATGTTTTCATTTGAAGCAATTTGTACTCCTCTAGTG13020.1103202846975089No Hit
CTCTGGTACTCCTTCCGTAGAAGGCCCTCTTTTCAGACCGCGTTTAAGGA12990.11006609049313676No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCGAGTGAGCAGGCA12860.10896458227419083No Hit
TCATTACTCATGTCAAAGGAGGGCACGATCGGGTTCGCTGCCCTTTCGTC12740.10794780545670225No Hit
GTTCTGGCCAGCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCGAG12670.1073546856465006No Hit
CTCATGGAATGGCTAAAGACAAGACCGATCCTGTCACCTCTGACTAAGGG12260.10388069818674801No Hit
CGATAAATGCATTTGAAAAAAAGGCGATCAAGAATCCACAATATCAAGTG11970.10142348754448398No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCACG100.0070859925145.03441145
CTAGACG100.007116598144.825494
AGGACGC256.4577343E-6116.02753145
GTGGTCC651.5625119E-978.09546145
GTCAATT950.076.3082
GTAGAAA8200.073.426481
GGGTAGA1900.072.5418241
TTAGTAG702.8012437E-972.5418241
ATGCGCC451.1925452E-464.45974145
CCATAGG1700.064.00751
TAGGGGC350.0035633162.157608145
CCGACGC350.0035633162.157608145
GATAGCA5700.058.440836
AGCGAAA502.0046686E-458.0334631
AATTATA1301.8189894E-1255.7021145
ATATTCA2400.054.3095634
GCCGACC553.215469E-452.739784145
GCTCGAC701.6937916E-551.798004145
AGGGTGT3700.050.886796
TCCCAAC4350.050.0288471
Produced by FastQC (version 0.11.5)