FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_H01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_H01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA12.7027027027027026No Hit
TCTTAGTTCTGATCCAGCTGCAAGTTTCCATGGGATTCTTTAATTTTTTA12.7027027027027026No Hit
GCTTCAATCCAATGATCGTCGAGCTTGCGGGAAAGGCAATGAAAGAATAT12.7027027027027026No Hit
CGGCTCCTGGTTCCCCACAGCTCGTCACAGTGATGTTGTTTTTCACGGGA12.7027027027027026No Hit
CTACAAGGACAGTCATGAGGCCCGACTGCAGGAGGCCGGGGACTACGTGC12.7027027027027026No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG12.7027027027027026No Hit
GACGCTACTTTTGCTAAAAATTCCTGCGCAAATTGCCTTAAGCAACACAT12.7027027027027026No Hit
GTTCAGGCACTTAGGGACAACCTGGAACCTGGAACCTTCGATCTTGGGGG12.7027027027027026No Hit
ATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTCTAGG12.7027027027027026No Hit
GCCATAAGCACCACATTCCCTTACACTGGATATCCTCCATACAGCCATGG12.7027027027027026No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT12.7027027027027026No Hit
CTTATACAATATACGGAATCTTCAAATTCCTGAAGTCTGCTTAAAATGGG12.7027027027027026No Hit
ATGAAGCCCCATGACCAACTGACGTCTGGCTAATAAAGGAAATTTATATT12.7027027027027026No Hit
CAACAATTCCATTTCTCCGTCCCCCCCCAGTCCCTGGCAACACCCAGTCG12.7027027027027026No Hit
GGCAGACAGAATGGCAGGCAGAGGCAGAGGGAGAAGCAGGCTCCCTGCTG12.7027027027027026No Hit
GCATGGCACCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGG12.7027027027027026No Hit
CCATATGGCCATAATCAAAGAAGACAAGAGATATGGCCCAGCATTAAGCA12.7027027027027026No Hit
GAAAAGGGAAAGTGGACGACAACCACAGAGACTGGTGCACCCCAGCTCAA12.7027027027027026No Hit
TCTCCTCTCCAGTGAATGAAAAGATGTGAATGTGTGTCTTCTCAGATTTT12.7027027027027026No Hit
TCCCTCGGCGTTGCTCCTGTGCACTCCTCTGCTCTGCTCAATGTGCTGAT12.7027027027027026No Hit
GGTGAACAGTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTGTTC12.7027027027027026No Hit
CAACATGCTAAGTACGGTCTTGGGAGTCTCGATACTGAATCTTGGACAAA12.7027027027027026No Hit
GGGCCATTGATCTCCCACATCATTGATGACGATTAAGTTATTGTCAACTT12.7027027027027026No Hit
ATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTAT12.7027027027027026No Hit
TGTATGGATTAACCAATTATATGCACAACCCAGACTGCCCAAGAACAGCT12.7027027027027026No Hit
ATTTTACAATCCTCGAGCTCTTCTTTCTTCTCTTTAGTTAATGCCAGCTG12.7027027027027026No Hit
CATATCATGATTTTGCATTCCAACCTCAAAGAAGCAACATATCAGAGAAC12.7027027027027026No Hit
ATACCTGTGAGAGCATAAACCGAGAAAAAGCCACTTTCAATGTATTTGTG12.7027027027027026No Hit
CTTCGAGAACAACTGGAGAATTTAATATGTGAGTCATCGGCACATAGGTT12.7027027027027026No Hit
TCACATTCCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTATC12.7027027027027026No Hit
ATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTAT12.7027027027027026No Hit
TGATAGGGAGGGCGCGAAAGCTGGAGAGCGAGAGGGAGGGAGCGCGAGCG12.7027027027027026No Hit
AAATTAAAGTTGCATGCATGAATGAATCTGAATTCATTTTAATTTTACAA12.7027027027027026No Hit
ATCTTCACATTCCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGAT12.7027027027027026No Hit
ATCAGGAACTGGGAAATTGTGAAAATTCAATGGTCACAAGATCCAACAAT12.7027027027027026No Hit
TAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAA12.7027027027027026No Hit
GTCTACGTGGCGCCCATTCACTGACATGGGTACTATTTTTTAAAGAGATC12.7027027027027026No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)