FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_G11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_G11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2817
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTA60.21299254526091588No Hit
CTTTTCAGACCGCGTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCG60.21299254526091588No Hit
ATGTATAGGCTACCATGCGAACAACTCAACCGACACTGTTGACACAGTAC50.1774937877174299No Hit
TGTCAAAGGAGGGCACGATCGGGTTCGCTGCCCTTTCGTCCGAGAGCTCG50.1774937877174299No Hit
GTTAGAGGTGATCTGAATTTCGTTAATAGAGCGAATCAGCGGTTGAATCC50.1774937877174299No Hit
GTCAATGAGGAGAATTGCATTCTACTTTGCTTTGGTGGAGCGGCTGCAAA40.1419950301739439No Hit
ATATAAATCCTGGCCATGCAGACCTCAGTGCCAAGGAGGCACAGGATGTA40.1419950301739439No Hit
GTATTAATGTATACCCCCTTCATTATATATTCTGTGGCTCTGCAATGAGA40.1419950301739439No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTCTCTATCGTCCCGTCAGGC40.1419950301739439No Hit
GAATGAGGAATCCCCTTAGAACAGCGGATTCCACTCCAGCTGTGCCTTCA40.1419950301739439No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCGAGTGAGCAGGCA40.1419950301739439No Hit
CCTTTAATTTCAATGTTTGCAGATTGCCCGTAAGCACTTCTTCCTCTCTC40.1419950301739439No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG40.1419950301739439No Hit
CGTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCGATCAAGAATCCA40.1419950301739439No Hit
CTATTAACGAAATTCAGATCACCTCTAACTGCCTTTATCATACAATCTTC40.1419950301739439No Hit
ACACAGGGAACATGCTGGGAACAGATGTACACTCCAGGTGGAGAAGTGAG30.10649627263045794No Hit
GTATAGCTGTTGCCCTTTTTCCTACTATTGTGAACTCTTCATATCCCTCA30.10649627263045794No Hit
GAACAATAAGGAGCAATTTTCTTGATTCAGCTGAAAATCCTTCTAATTGT30.10649627263045794No Hit
GTTTATAGCTCCCTGGGGTGTTTGACACTTCGCGTCACATTCATCCATTG30.10649627263045794No Hit
ATATTCTACACTGCAAAGACCCATTAGAACACATCCAGAAGCTGATTGCC30.10649627263045794No Hit
CATATACAACAGGATGGGGGCTGTGACCACCGAATCGGCATTTGGCCTAA30.10649627263045794No Hit
AGTAGAAACAAGGCATTTTTTCACGAAGGACAAGCCAAATTCCCTATTTT30.10649627263045794No Hit
CTTAATATGAGTGCAGACCGTGCCAGAAAGGTGAGATCTTCGATCTCAGC30.10649627263045794No Hit
CCTCTGGAGACAGTAGTACATTTCCTCTTTGGTCCCGAACTCTCAAAAAC30.10649627263045794No Hit
GTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGAGTG30.10649627263045794No Hit
GCATAAACGCCCCTGGTAATCCTCATCCATCAATTCCCATTTCAAGCAGA30.10649627263045794No Hit
TTCTTCACATTTGAGTCATGAAAATCCAAAGTCCTTTCATTTTCCAGTAG30.10649627263045794No Hit
GCATATGTCCACAGCTTGCTCTTCTGTTGGATTTTGTCTAAGAATGTCTA30.10649627263045794No Hit
GATGTACACTCCAGGTGGAGAAGTGAGGAATGATGATGTTGATCAAAGCT30.10649627263045794No Hit
CTATACAATAAAATGGAATTTGAGCCATTTCAGTCTCTAGTTCCTAAGGC30.10649627263045794No Hit
CTTCTGGAGGGGTGAGAATGGAAGAAAAACAAGGATTGCTTATGAGAGAA30.10649627263045794No Hit
GTCTCGCACTCGCGAGATACTTACAAAAACCACCGTAGACCACATGGCCA30.10649627263045794No Hit
ATTGTGAACTCTTCATATCCCTCATGCACCTTTAATTTCAATGTTTGCAG30.10649627263045794No Hit
CCTTACAGCCATGGGACAGGGACAGGGTACACCATGGATACAGTCAACAG30.10649627263045794No Hit
AATATGGACAGAGCCGTTAAACTGTATAGAAAGCTTAAGAGGGAGATAAC30.10649627263045794No Hit
ATCACTCACTGAGTGACATCAAAGTCATGGCGTCCCAAGGCACCAAACGG30.10649627263045794No Hit
AGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGCTGACAAAATGACC30.10649627263045794No Hit
ATGCTATACAATAAAATGGAATTTGAGCCATTTCAGTCTCTAGTTCCTAA30.10649627263045794No Hit
ATTACAAGGAGAATAGATTCATCGAGATTGGAGTGACAAGGAGGGAAGTT30.10649627263045794No Hit
GCCCAGTACCTGCTTCTCAGTTCAAGAGTACTTGATACCATAGTATCCAT30.10649627263045794No Hit
TCATATCCCTCATGCACCTTTAATTTCAATGTTTGCAGATTGCCCGTAAG30.10649627263045794No Hit
TTTCTGATCTATTCTCAAAAAACTCTTTTGTCATGTCTTTCTCCTTGACA30.10649627263045794No Hit
GGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCT30.10649627263045794No Hit
CTATTAATAGATGGAACTGCATCACTGAGCCCTGGAATGATGATGGGCAT30.10649627263045794No Hit
CCCATAAGGGGAGAAGCAGTTTTTACAGAAATTTGCTATGGCTGACGGAG30.10649627263045794No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCTGCTGT30.10649627263045794No Hit
GGATGAATGTGACGCGAAGTGTCAAACACCCCAGGGAGCTATAAACAGTA30.10649627263045794No Hit
GTGTTGGAAAGCAAATAGTGGAGAGGATTCTGAAGGAAGAATCCGATGAG30.10649627263045794No Hit
GTATGATTGAAGCTGAGTCCTCTGTCAAGGAGAAAGACATGACAAAAGAG30.10649627263045794No Hit
GAATAAAAGAGCTAAGGAATCTGATGTCGCAGTCTCGCACTCGCGAGATA30.10649627263045794No Hit
GTAGTACATTTCCTCTTTGGTCCCGAACTCTCAAAAACCGGTCAATGCTC30.10649627263045794No Hit
ATTACATCCTGTGCCTCCTTGGCACTGAGGTCTGCATGGCCAGGATTTAT30.10649627263045794No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGG100.006958328143.321438
Produced by FastQC (version 0.11.5)