FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_G07-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_G07-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences998309
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA14910.14935255517079382No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT14650.14674815112354994No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG13790.1381335838903586No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA10550.10567870268624244No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGCGG750.001488035438.6420443
CGAAAGC3350.034.6048163
TATACCG1102.448151E-432.9269645
AGCGAAA3450.031.5490861
TATACAG10400.031.3439335
ATATACT11600.029.976394
TATATAC23600.027.6306973
TATACTT12700.027.378645
ATACTTA12450.026.7620456
GTTACGC3255.0931703E-1126.7521863
CAGCTCG11500.026.4560959
GCGAAAG4250.025.5975912
GTATATA21800.024.6314431
CATATAT13800.024.1876321
GTATAGT2751.952194E-723.7478581
CTTACAC12300.023.55759
TACTTAC12750.023.2930437
TTACGCA3503.5342964E-922.7677824
ACTTACA13200.022.4989628
ATATATA15250.022.3524322
Produced by FastQC (version 0.11.5)