FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_G06_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_G06_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1708
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAACACAAGGTGCCTAGAGTCCTCAAGCTCATAGAGACAGAGAAATA20.117096018735363No Hit
CTCTAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCT20.117096018735363No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.117096018735363No Hit
CACTTGGGCTAACCCCCACGTCAGCAAACCCAGACTGAACAGCTAACCGA20.117096018735363No Hit
AAGTAGTGTAGAGGATGGCTTGGAAAGGGAGGGACTGGCCGTACAAAAAG20.117096018735363No Hit
CTCTTTCAGAGGACGGTGCACTTGGGAATGCAGGTACTGATGCTGAACTG20.117096018735363No Hit
GGCTGGGAGAATGCTGGCTGTACGGAAAGGCTGGAGCTGACCACGTGGTG20.117096018735363No Hit
GAGAAGGTGTTTCAGTACCTGGATCGGAAGCCAAAGCTGCCTCCGCCGGG20.117096018735363No Hit
ATCTAAGAGTATCAAGCTTCATCAGAGGGACAAGAGTACTTCCAAGGGGG20.117096018735363No Hit
GTATTGGTCAATACCTATCAGTGGATCATCAGAAACTGGGAGACTGTTAA20.117096018735363No Hit
GACCAGGTCTCTGTCATCAAGGTGAAGATTCACGAAGCCACGGGTATGCC20.117096018735363No Hit
CCCCTGAAAGTTATTGTTACAAGGAAACAGCAGCCCAGGAGCAGTCACTT20.117096018735363No Hit
GAGGGAGGGAGAGAAAGAGGATTTCAAGCAAACGTCCCTCTGAGCAAAGG20.117096018735363No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAAA100.006919807141.882351
Produced by FastQC (version 0.11.5)