FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_G06-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_G06-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865662
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA9670.11170641659215721No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2050.049.547431
TATACGT2150.047.1555674
CAGCTCG6800.030.8839269
TATACAG8000.030.7774285
GTATACG2901.2732926E-1129.9762463
TCCGAAC1453.517091E-529.9658554
CGTGCTA3001.8189894E-1128.9669918
GTACTAT2552.9995135E-928.4516051
CGAAAGC3900.027.8625343
GGTCCGA1606.828975E-527.1863962
TATATAC16450.026.4228293
GTATAGG1658.37664E-526.3823991
GTCTAGG1958.704494E-626.0441591
ATATACT8800.025.5107044
CTAGGCT5200.025.067594
GTATAAG2901.1812517E-825.0177921
TATACTC2051.3014394E-524.7279195
GTATATA14000.024.3566061
GTATAAA6400.022.6723731
TATACTT9400.022.3415625
Produced by FastQC (version 0.11.5)