FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_G05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_G05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCATGGGGGCGTATACCCGGGAGGTGCGCAGCAGCTGGGGGGCCAGGC12.941176470588235No Hit
ACCCAGCTTTGGAGTGTCTGGAGTAAATGAATCAGCTGAAATGAGTATTG12.941176470588235No Hit
GATATAATAGTAAGAAATTAAAAAAGTTTGGAGATTGTTCATAATAGATC12.941176470588235No Hit
ACTAATCAGCATCCCATGTGTTGCGATAACCCTTCTTTTTTAAAAGCATC12.941176470588235No Hit
CTATCAACTGGATCAATCTCCTGGTTGCCTTTCTGAGAATAGCTGTTGCT12.941176470588235No Hit
ATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCA12.941176470588235No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG12.941176470588235No Hit
CATTTGGAAGTTTGTTTCATGTATTCAGATTTACATTTCATCTACGAACG12.941176470588235No Hit
GACAGAAGCTGGAGATTTGACAGCTTATCCCAATGAGGCCCATCAGGCCA12.941176470588235No Hit
GTACTATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGAACTGAGAAGTT12.941176470588235No Hit
ACTCAATATTAGAGTACATTACGTGTATGAACAATGCAAAATTGGGATCA12.941176470588235No Hit
GAATGTAGGCTGCACACTGATCTGGCCTGCGGATGCCTTTTGTTGATTGG12.941176470588235No Hit
ATTACAGCATGCCGATTTAACATAATTTTATTAATACAATTCCTTGTAAA12.941176470588235No Hit
GACCCATATATAGAAGAGTAGACGGAAAGTGGATGAGAGCCCTCATCCGT12.941176470588235No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGCTCGAAGGTTCCAGGTTCCA12.941176470588235No Hit
TAAATGGGGACAACAATAATGTTAAGTAAAATAAAAATCATCGAATTTTA12.941176470588235No Hit
TGCTATGGCAGACGAAGAAGCATCGCTCGTACCCAAATCTGAGCAAGACA12.941176470588235No Hit
CTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAG12.941176470588235No Hit
ACCCACTGCAGCAAAACAAACGATTCTGGATCAGACAGACAGATCGTATC12.941176470588235No Hit
CCTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCGATCAAGAATCCA12.941176470588235No Hit
GACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGGGCTATCGCAACAC12.941176470588235No Hit
CGAATAAGTTATTGTCAACTTCTCAGTTCCTTGCGTTTCACTGACTTCTT12.941176470588235No Hit
AGTCTCTTCAGCAGATTGAGAGCATGATTGAGGCCGAGTCTTCTGTCAAA12.941176470588235No Hit
CTTCATACCCTTCATGTACTCTTATTTTCAGTGTTTGGAGGTTGCCGGTT12.941176470588235No Hit
GCACCAACTCTTGAGGCATTTCCAAAAAGATGCAAAAGTGCTTTTCCAGA12.941176470588235No Hit
GCAAAAAGAAGTCCTATATAAATAAGACAGGGACATTTGAATTCACAAGC12.941176470588235No Hit
GCACTTCTTCTTCTTTCTTGACTGATGATCCGCTTGTCCTTTTGAAAGTG12.941176470588235No Hit
GCACTTAACCCAAGGGACGTGGTGGGAGCAGATGTACACTCCAGGAGGAG12.941176470588235No Hit
CATTATTTCTGAGGAAAGCGGCTCAAAATCTCTATACTGATTTGCACAAA12.941176470588235No Hit
GAACATGCCCATCATCATTCCAGGGCTCAGTGATGCAGTTCCATCTATTA12.941176470588235No Hit
CAACTATGCAGGAATTCAAGCTGGAGTTGACAGGTTTTATCGAACCTCTA12.941176470588235No Hit
TTTGAATTCACACGCATTTTTTATCGCTATGGAGTTGTGTCTAATTTTAG12.941176470588235No Hit
TATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTGA12.941176470588235No Hit
TGGGAGCCGCAGATAGACGGTGGCATACAGATTTTGCCAGTAAGGGCCTG12.941176470588235No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)