FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_G05-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_G05-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences975183
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAAATCCTGGCCATGCAGACCTCAGTGCCAAGGAGGCACAGGATGTA11920.12223346797472885No Hit
CATTAATACTGCTTTGCTTAATGCATCCTGTGCAGCAATGGATGATTTCC11250.11536296264393453No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCTGCTGT11180.11464514865415004No Hit
CTCTAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCT10050.10305757996191484No Hit
ATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGA9830.10080159313687789No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1200.090.7242661
GTAGAAA6750.046.2120252
CAAGGTA2700.045.604429
TATGCCG657.369347E-444.574935
TAATGTA4250.035.7910465
ATATAGT4700.035.5175821
ATATTCA3950.034.852524
ATCGTGT1301.6656286E-533.429489
TGTCGTA1751.0040094E-733.162122
GTATACC4400.032.9229779
GTATTAA4850.032.922621
GTCAATT1551.458322E-632.7609672
TATCGTG1352.1562313E-532.1913578
GGACTTA1153.1798644E-431.4915456
GTATTAT2558.731149E-1131.3087651
GTGGTAT5150.030.9412026
CAAGGGT4950.030.7391454
ACTATAT4650.029.6150173
GTCTGCG1000.00606780829.006428145
ATATCGT1555.536319E-528.037637
Produced by FastQC (version 0.11.5)