FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_G04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_G04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTGGGAGAAGAGCAACAGCTATTCTCAGAAAGGCAACCAGGAGATTGA11.2658227848101267No Hit
GATAAATACTAAAAGTGTAACGGTGGCAGCAGATAAATGGTGAAAAGAGA11.2658227848101267No Hit
GCAGAAAAGCAAAATAATTAATACCATCAAATTTGAGACTGCTAATATGG11.2658227848101267No Hit
CCTTTGAAATTTCCCAAGCATAACCCTAAATATGGAGAACACATAGGTAT11.2658227848101267No Hit
GGCCAGCGTAAAAGGATATGGAAAAGGGACCGGAACTCTCAGCTCTAAAT11.2658227848101267No Hit
AATCTAACATACGACATACAAATGCGTTAAATATATGCAATCTGGCCAGA11.2658227848101267No Hit
ATATTGAAGTGTTGCACTTAACCCAAGGGACGTGCTGGGAGCAGATGTAC11.2658227848101267No Hit
CTCTTATTCAGACTTTGTTTGTTGTTCCCTATTGTTCGTAGCGTGACCAG11.2658227848101267No Hit
GATAATCACTCAATCAGTGACATCGAAGCCATGGCGTCTCAACGCGCGAA11.2658227848101267No Hit
ATCAGCTACTCATCAAAAATGGAAGAATATGTGAGAAAATGATTAAATAC11.2658227848101267No Hit
GAGAGAGAGAGAAAGAGATCACAAGTAGGCAGAGGCAGGCAGAGAGAGAG11.2658227848101267No Hit
TCATATGCCTCTGCTCTCCACGTCCTGATCACCCCATATGTGAACTACCA11.2658227848101267No Hit
TGTCTGAACGGCTCCTAAGCATGTAACTCATGACTAAAGACACTGCACGG11.2658227848101267No Hit
TGCTGGTTTCGAGCGACACTGCAACCAATAGAGGTAAGGAGTGGATAAAG11.2658227848101267No Hit
AACCAGAGTTACAGTGTCTTTTGTCCCTGAAGCAGGGTCTCCCCGTAGCT11.2658227848101267No Hit
GTTGCATCTGCACCCCCATTCGTTTCTGATAGGCCTGCAAATTTTCAAGA11.2658227848101267No Hit
GTAGTAGCTCCTGGGCTCTGTTGGAGCGGGGAGGTTTTGCCCCCCATGGG11.2658227848101267No Hit
GTGCGTGTGTGGGTTGTAGGCTGCAGAATCACAGAGAGCCTGGGCTCCTG11.2658227848101267No Hit
GCCCTGACAGGAATCCCAAGAGGGTGTGGTTTGGCTACCTAGGTGCTGGT11.2658227848101267No Hit
TCTTTTTTCCAGGTGGTTGAACTCTTTACCTACTGCTGTGAACTGTGTAT11.2658227848101267No Hit
GCTTAAGCTAGTTGTTATCCCAATAGAAGTCGGCCGTAGTTTTCAGAGAT11.2658227848101267No Hit
GGCCATGGAGATGGTGGGCTTCCTCCCTGCCACCAAGAAGCAGATTTTTT11.2658227848101267No Hit
GATATTAACTATGTTAGGATGTAATTTTGTATATGTATATTTTTGCCCTT11.2658227848101267No Hit
GTTTCCTCCATGGGGGAATTGCCCGGGACGCGGGGCGCCAGCAGCAACAG11.2658227848101267No Hit
AACATGTATGCAACCATCAAAGGGGAAATTTTGCATTTCTGGAGTTCGTG11.2658227848101267No Hit
GGTGTCATGTGAGGCTTTTTTGGTTGGGGATCGGTGTCCTTTGAGATTTA11.2658227848101267No Hit
ACACAGGACATGCTGCTGGAGATGCTTGAGGACATGACTACAGGTACAGA11.2658227848101267No Hit
GAAGCCCTTGAGGATCATCAACCTGAACTTCTGTGAGCAGGTGGATGCGG11.2658227848101267No Hit
GTTGTACTGAATCCCATTCTCGCCCCTTCCCGCCATCCAAAAGAAAGGAA11.2658227848101267No Hit
CCTCTAAGCAGCAGTTTTTAATGAAAATGACAGACCTGCAGAGAAAGAAG11.2658227848101267No Hit
CAATAATATAATCAACCACTCCCGACAATACATCAAACCGTGATAAACAC11.2658227848101267No Hit
TTAGATTGTTATCGGGATACGCAATTAACAATATTAGCCTGGGGGAGAAC11.2658227848101267No Hit
TCCTTGATCTGGTATTTCACTTTCCCGGCGAAGTGCTGGATGATGAACGC11.2658227848101267No Hit
GTACCAGCGTAAGTCGGGGGGGAGGTAAGGAGGCTGTTGTGACAAGCAGC11.2658227848101267No Hit
CCCTAGGAGATCTGGAGCTGCTGGCGCTGCAGTCAAAGGAGTTGGAACAA11.2658227848101267No Hit
AGACTGTACGTTGTAGTGCGTCCAGACAGTGGACTAAAAAGAAATGAGCT11.2658227848101267No Hit
CTTAAGCGGGAAGAAAATGTTGACACCTGCTGTACAACAGGTGAGCCTTG11.2658227848101267No Hit
GAATCTATGGATATCCAGCAGACTGTATGAAAAATGATGGTAAGGCAGTC11.2658227848101267No Hit
GATGATTTGGTCATGCAAATCGCGGTAGAGTTTTTCTGAAGGGGTGCTCG11.2658227848101267No Hit
CAAGAAGATTGTATGATAAAGGCAGTTAGAGGTGATCTGAATTTCGTTAA11.2658227848101267No Hit
AAGCTAGGATGTGGAGGGGTAGAACACTGTTTCCTAATTAACAGTGTTAA11.2658227848101267No Hit
TCTTAGACGGGGATCATGGGGGGGAGAAATACACGGCGGATGTGATTCTA11.2658227848101267No Hit
TTGGGAGCAGCGCGGCTTCCTTCATCTCAGTATTTGAGGTATGTTTTGGT11.2658227848101267No Hit
GGGAGAGGGCAAGGCAGGCTCCCTGAGATCGTGGCCTGAGCCGACGGAGC11.2658227848101267No Hit
GTACAGATAATAATCATCAACCATAAGAAATTCCATAAAAGGATTTGTAG11.2658227848101267No Hit
ATATACAGTTAAGCAAAATTGTATGTAAAACCAGTTTTAGAAATTGGGTT11.2658227848101267No Hit
AGAAAGAAGGATCGAACACGTAAATAAAAAAGTTGATGATGGAATTCTGG11.2658227848101267No Hit
TCTTAGAAGCATATCTCCTATCTCAAGAACACAGTACTTCTCCCATTTGT11.2658227848101267No Hit
GTAGCATCGCTCATTCGCTCCTAGGTTCGTTCATTCATTCACTCACCCAC11.2658227848101267No Hit
GTCTGTGGGGTGTGTCGCAGCGCTCTGGCCCCTGGCGTCCGAGCTCCGGA11.2658227848101267No Hit
GTCAAGAACCGGAGCAACAACAGTCGTAAGGCCAGGGGCGAGAGCGACAC11.2658227848101267No Hit
CTGCACAATTTGTACCAGCCCACTGGGGGACGGCTGCTGCTGGATGGGAA11.2658227848101267No Hit
GGCAGAGGCAGAGAGAGAAGCCTGCTACCCGCTGAGCAAGGAGCGTGATG11.2658227848101267No Hit
ATCCTGAACTGGCGGGTGTCGGCCCAATTTCAAATATTCTGTCTGTTGTT11.2658227848101267No Hit
TTACAAATCAGAAGTCATCTTGCTACCAGTATTTAGAAGCCAACCGTGAT11.2658227848101267No Hit
GTATTCTCTCAGGAGGAAAGCATGCTCAACGCAGATAGGCGCCAAGTGAA11.2658227848101267No Hit
GTACATGAAGGGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGC11.2658227848101267No Hit
TCGTGGCACGGGACTTCGAAACATAATTTGACCGCATCCAAGACCCCGAA11.2658227848101267No Hit
AAGGAGGAGACCATTTGGAAAATGTGAAAAATATTCTTGGGTTTGATCTG11.2658227848101267No Hit
GTCCGTGATTGCCGCTGTGTGTGCGCACACGATCTGTGCAGACGTGCGGC11.2658227848101267No Hit
CATCTAGACTTGTGCATCCCTAGTGTTCAAGGCCGGTCACTCAAACTTGA11.2658227848101267No Hit
GAGTCTAAGGAATCTACTTGTTCAGGCGCTTGTAATTTGACTGAGCAGTT11.2658227848101267No Hit
GTTGAGCTCTGTCCCCGGTGCACTTTAGCACTGGCGGTCGGACACGACTT11.2658227848101267No Hit
CATCCCAGGCCTCAGTGCTGCTGTGCCATCTATTAGAAGAGGCCTGATTT11.2658227848101267No Hit
ACTGAATCCCATTCTCGCCCCTTCCCGCCATCCAAAAGAAAGGAAAAAAA11.2658227848101267No Hit
CTTCAGCTCCGCTAAAAGTCACATGACATAGACGTAGCGATTTGATTCTG11.2658227848101267No Hit
CATTAGTGTTGTTGATATTAACATATGTTTGATTTACCCAGGTGCTATTT11.2658227848101267No Hit
GACCTGACCCCGAGGACAGCGGGTGGTGTTTCCCCACAGATGGTCCCAGT11.2658227848101267No Hit
CATCAGTACTCAGAAAGAGGAAGATGGACAAAAAATACCGAAACTGGAGC11.2658227848101267No Hit
GCCCCAGCCCTGCATCTCGGATCGTGATTCCTCATTTAGAAGGAAGAACC11.2658227848101267No Hit
ATTTGGAGGCAGGGTCTTGGGTAGATAGTTAGCTTTAAAGGCAGTCCGTC11.2658227848101267No Hit
GGCTTTCACTATCTAATATATCTGGAATTCACTCAGACTTACTCCAATAT11.2658227848101267No Hit
GTCCAGGAAGACTTATCATCGCCACTGTGGTCTTCATCGTACTTGTCAAA11.2658227848101267No Hit
GTCCTGCTATTCTTGATCGCAGAGAAGTCTTCTTTCGTGATACGTCCGGA11.2658227848101267No Hit
ATTCCTTCTTTACTTCTCCAATTGTTCAATGGAATTAATTAACTGTAGCT11.2658227848101267No Hit
CACTGGCGCCGATGACATACTATATCAACCCTTCTATGTTGTTCAACTTC11.2658227848101267No Hit
TTCCAGAACTGGGGAATTGAATCCATCGACAATGTGATGGGAATGATCGG11.2658227848101267No Hit
AGATAGAAGAGCCCGGTGGGCTTCCTGCTGATGAGATGGATGCAGCCGAC11.2658227848101267No Hit
GTATTGACCGATTTTTAAGGGTTAGAGATCAAAGAGGGAACGTACTATTG11.2658227848101267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGAGC50.0145.01
AGCTCTG50.0145.05
TCTGTCC50.0145.08
GCTCTGT50.0145.06
CTGTCCC50.0145.09
AAGTCTT50.0145.0145
CTCTGTC50.0145.07
GAGCTCT100.072.54
TTGAGCT100.072.52
TGAGCTC100.072.53
Produced by FastQC (version 0.11.5)