FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_G03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_G03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTGCAATCGTGGACTGGTGTATCTGAAATGATAATACCAGATCCAGC12.7027027027027026No Hit
TTGGCATACACTTGATCGCATCATATAGTGGGATTCCCTCTCCTTCATGA12.7027027027027026No Hit
CTCACGCACCCTGTGTACTCTGATCTCCGGCGGTAGGCCATGGGCACAGG12.7027027027027026No Hit
ATAATGCAGTAATAAGCAACCTCCTAAATAGGCAACGGTCTCTGTTACTC12.7027027027027026No Hit
CACTGAAATGGCTCAAATACCATTTTATTGTATACCATTGTAGGGTACAC12.7027027027027026No Hit
GCTCAATACTCACTACCACTCTCTCCGTGCTGGAGTATTCATATACTCCC12.7027027027027026No Hit
GTACTCCTCTAGTGGAAAGCTTCCCCCTTGGAAGTACTCTTGTCCCTCTG12.7027027027027026No Hit
CTCCACCTCTGTCTGAGGGGGACGCCTGATTTCCGCCCAGTCCCCTGAGA12.7027027027027026No Hit
CTCTTAGATCTTCAAATGCAGCAGAATTGCATGCCATCCACACCAACTGA12.7027027027027026No Hit
ATCTCGCCGGGCAATTTGAACCAATCCAGCAGAGCCTCATTAATGAACCA12.7027027027027026No Hit
AATTAACTGTAACATTCTTCTCATTAACCACCCCCCCAGTTCTCCAACCT12.7027027027027026No Hit
GAATAAGCTTGGGATATTTCTGTGTTGAACTATTTCCATGTCCCAGTTTA12.7027027027027026No Hit
GGGTGTGCCCTTGAGACAGACTTTACATCTACCCCAGGAACACTCAGGAT12.7027027027027026No Hit
ACATGATATGATCCTAGAACTCTCAGCTAACGTGGTCTAATTCACGCAAG12.7027027027027026No Hit
GTTCCATTTTTCACACTTTCCATGCATTCATTGTTACACTTGTGGTAGAA12.7027027027027026No Hit
CAATCATACTCTCTATTTGTTGAAGTGATTGGAGGAGGCAACGCCTCATC12.7027027027027026No Hit
CAATTAAACCTGACCATTCAACATTGTTCACCTTAACAGACAGCATTGAG12.7027027027027026No Hit
ATTCACACACACACAAGTTTCTAATATCAGATGTCCATTCACCACTATCA12.7027027027027026No Hit
GTAGACACAAGGGACTGGTTTGGACAGTAGGGCTAGCAAATAGTAGACTT12.7027027027027026No Hit
GGTCCCAGGTTCAAGATTGTCACTAAGCGCCTGCAAAATAAGGAGCAATT12.7027027027027026No Hit
CTGTAGTGGCTTCACAGTATAACAGGAGATGCACCCCAGAAATAGAAACA12.7027027027027026No Hit
TTCTAACACTTACACTTAGTGACACAGGTGCCAGGTGACAGAGCAGAAAG12.7027027027027026No Hit
GTTCCTGACACCCCCAGGCCCAGAACCGAAGGTGACCTCTGCGGTTTTCT12.7027027027027026No Hit
ATACCTATAAGTGGATCATCAGAAACTGGGAGAATGTTAAAATTAAGTGG12.7027027027027026No Hit
CTTCAAGAGTTTGAATTCTTTGGGCACCACTGATCTCAGTCTAGCCACGT12.7027027027027026No Hit
TACTGCAGCTCTTCTCACTATGGTCCTATCAGCAATAATTAAGCTTTGAT12.7027027027027026No Hit
GTCTTCAAGTTTGTTTTTGCCATCTTCAATTTATTTCATCAGTGTATACT12.7027027027027026No Hit
TCAGAATAAAACATCATATAAGATTTCCTATTCCTATAGAAATCTCAATC12.7027027027027026No Hit
AAACTGCAATTAGTTGATATGCTGAAAAGCAAATTATGTTTTTATGATCT12.7027027027027026No Hit
GGTTGATTAATACATTGCCCGAGCTGCCATGAAGCCGGGGGGCGGGGGCG12.7027027027027026No Hit
GTGAGAAAGATGATGACTAATTCACAAGACACAGAGATTTCTTTCACAAT12.7027027027027026No Hit
CACACATGCAGTCACGAAGTAGTGTTGACCAAATCTCCCACAAAGTAAGT12.7027027027027026No Hit
GATCTATCCAGCTTGAATAGGTCAGCTAGCATGCCTTGTTGAACTCATTC12.7027027027027026No Hit
CTGCAAAGACCCATTAGAACACATCCAGAAGCTGATTGCCCCCAGGGAGA12.7027027027027026No Hit
CCATAGTGATTGCCCTTGCTCATATCTCTCAGCAATCATTTCCGTTATCC12.7027027027027026No Hit
CCTTGTGTTAAGTAGAATAACAAAGTATTTCTTTTCGCAACTGGCATATC12.7027027027027026No Hit
CCTTGGCACTGAGGTCTGCATGGCCAGGATTTATATCGACTCTTCGGCGT12.7027027027027026No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)