FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_G02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_G02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1244
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTT40.3215434083601286No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA30.2411575562700965No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC30.2411575562700965No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG30.2411575562700965No Hit
ATGTTGTTCTTTATCACTGTTACTCCAATACTCATGTCAGCTGATTCATT30.2411575562700965No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA30.2411575562700965No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG30.2411575562700965No Hit
TATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCTGG20.1607717041800643No Hit
ACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAG20.1607717041800643No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA20.1607717041800643No Hit
ATCTCCTGGTTGCCTTTCTGAGAATAGCTGTTGCTCTTCTCCCAACCATT20.1607717041800643No Hit
TAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAA20.1607717041800643No Hit
GAATATGATGCCGTTGCAACTACACATTCCTGGATTCCCAAGAGGAATCG20.1607717041800643No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT20.1607717041800643No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG20.1607717041800643No Hit
TTGTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTG20.1607717041800643No Hit
ATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAA20.1607717041800643No Hit
CCTTTCGTCAGTCCGAAAGAGGCGAAGAGACAATTGAAGAAAAATTTGAG20.1607717041800643No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG20.1607717041800643No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.1607717041800643No Hit
GCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTGCCTGA20.1607717041800643No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCC20.1607717041800643No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT20.1607717041800643No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT20.1607717041800643No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA20.1607717041800643No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC20.1607717041800643No Hit
TTATGGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG20.1607717041800643No Hit
CCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCA20.1607717041800643No Hit
GGGGAGGGAATACCACTATATGATGCAATCAAATGCATGAAGACATTCTT20.1607717041800643No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA20.1607717041800643No Hit
GACTAGGGAAAGGGTACATGTTCGAGAGTAAAAGAATGAAGATTCGAACA20.1607717041800643No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC20.1607717041800643No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA20.1607717041800643No Hit
GGGGAGTGGAGGAAGGCTCTATTGGGAAAGTGTGCAGGACCTTACTGGCA20.1607717041800643No Hit
CTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGT20.1607717041800643No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC20.1607717041800643No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG20.1607717041800643No Hit
GAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCA20.1607717041800643No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC20.1607717041800643No Hit
GCAATTGACTGCTCGTCTCTCCCGCTTACTATCAACTGGATCAATCTCCT20.1607717041800643No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC20.1607717041800643No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA20.1607717041800643No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT20.1607717041800643No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC20.1607717041800643No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG20.1607717041800643No Hit
CAATATCAGGTGCAAGATCCCAATGATATTTGCTGCAATGACGAGAGGAT20.1607717041800643No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG20.1607717041800643No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)