FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_G01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_G01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG27.6923076923076925No Hit
GAGATATGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGA13.8461538461538463No Hit
CAAGAACGCTGTGGATATCCGCGTTGGATATGTGTACGGAAGTCTGAAGC13.8461538461538463No Hit
CTTAATTTCAGAGCATCCATCAGCAGGAACTTTGACCGCTGATGGCAAAG13.8461538461538463No Hit
GGCGGGGGGCCAGGAAGGAAATACGGCGTAACGTCACTAGGAACTGTAAC13.8461538461538463No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC13.8461538461538463No Hit
CTTTCCCAATAGAGCCTTCCTCCACTCCCCTGGGTGACTCTCCGATTGGC13.8461538461538463No Hit
TCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATACA13.8461538461538463No Hit
GAGGCAACACCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAA13.8461538461538463No Hit
ATCAAATATTTTACACAAGATATTTCAAAATATTCTAGAAAGTTATTTTT13.8461538461538463No Hit
GTGCATGGGGTTCAGTCGCTGGTTTGCCCTATTGACAAAGTTCAGATCGC13.8461538461538463No Hit
AGGCTACGTCAGTCCAGCTTCCCTGGGTGTTAAACAACACAATTTTCCTC13.8461538461538463No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC13.8461538461538463No Hit
GGGCCCAGCTGATGGGATGGGGATCCTCACCAGGGTGTCAGAGAGAGATT13.8461538461538463No Hit
GTATCGACATGATCACCTGGGTTTATTTCCCTTATTGGCAAAAGAGACTG13.8461538461538463No Hit
GAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAG13.8461538461538463No Hit
TCCAGGAAGAGAATGCTTGCTGCAGGTTGGGGAAGAGACAAACACAGGCC13.8461538461538463No Hit
CCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAAAC13.8461538461538463No Hit
AAAGGAGGAGGAGTGGGAGAGGGATAAGCTGGCTTCTCACTGAGCAGGGA13.8461538461538463No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC13.8461538461538463No Hit
CAGCTGAGCGGGGGATGGCTCCCACAGCGATCCACCAGCAGCGCCAGGAA13.8461538461538463No Hit
GAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAAACCCCTCAAGTTGT13.8461538461538463No Hit
GGAACAGGATACACCATGATGCCGTTGCAACTACACATTCCTGGATTCCC13.8461538461538463No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC13.8461538461538463No Hit
CTTCAGTGCATGTGTGAGGAAGGAGTTGAAACAAGATGCATTAAGCAAAA13.8461538461538463No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)