FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_F12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_F12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAAACACATACAGAAGAGACACAGCCGGAGGACGTTCCAGCCTACCAC16.25No Hit
TTCCCAATAGAGCCTTCCTCCACTCCCCTGGGGGCCTGTCCGAGTGGCCA16.25No Hit
CTAGAACAGTCTCAAGTCATTCTCAGGGATGATGTCGTAGTTGTGCCTCG16.25No Hit
CTTTCGGACTGACGAAAGGAATCCCATAGACTCCTACTGGCCAATTCTTT16.25No Hit
CCTGGGGATAATGAACCAAGTGGGTATGCACAGCCTGTGTTCTAGAGGCT16.25No Hit
CTAAGTGATGGCCATGGGAAGTCTTGTGGTGGCTGGGTGGGGGTCGGGAA16.25No Hit
NNGCTGAACACCGCTGTTGTATGGCTGGTGATAGAGTTCCGAATCGACCG16.25No Hit
GCCCTCAAGTTGGCGAGCCGCAGATAGACTGGAGCAGACAGATTATGCCA16.25No Hit
GTCCAGGGCCCACAGCCCCAAGTACCTTCAGTTCAAAGGGAAGCTGGCAC16.25No Hit
CTATTCGACACTAATTTATGGCCATCCGAATTCTTTTGGTCGCTGTCTTG16.25No Hit
GGTAGGATGTCACGCATGCAGGGGTCTTAACACCTCCAGCAAGCCCCACT16.25No Hit
ATCCTGGGAAATACAGAGTGTGAATCACTCTCCACAGCAAGCTAATGGTC16.25No Hit
GAAACAAGGAGGGGAACCAAAGGTCGAGGTGGCAGAGGGGAAATGAATGG16.25No Hit
ATCAGGAACTGGGATATTGTGAAAATTCAATGGTCACAAGATCCCACAAT16.25No Hit
CAGCCTTTCCGTACAGCCAGCATTCTCCCAGCCTGCCTGACCTGGGCTGG16.25No Hit
CTCTCCAATCAGCCACAAACCCATCCCTCAGTACAACAGGCCCAGCCCAC16.25No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)