FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_F11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_F11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1544
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT40.2590673575129534No Hit
GACCTGCATGGTGGGGGTGATATTGGGAAGGGAGACACGTCAGATCCCCA30.19430051813471502No Hit
GGGCAGTTGGACCTGGAGCAATTTTCTGGTCTTTGGGGGAGAGGGGATGG30.19430051813471502No Hit
ATCCATGAGCATGGATTGTCTTCCCGTTTGTTTGTGCCATCTTCAATTTC30.19430051813471502No Hit
GGCTGGAGCTGACCACGTGGTGGAAAGAGCCAGTTTGGGAACTAGGTGTC20.1295336787564767No Hit
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA20.1295336787564767No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT20.1295336787564767No Hit
ATTTTACCTAAACGCTTAGAGCCACAGCGTAAGCTAAAAACCAGGAGCAG20.1295336787564767No Hit
CAATAAAACCGTGTGGAAGACTGTGTTCAGTGGCATACACTAAATGCAGA20.1295336787564767No Hit
GAATATAGAAAATAAGGCTGGGGCGCGTAGGTGGCTCAGTCATTAAGTGT20.1295336787564767No Hit
GTGTAGAGGATGGCTTGGAAAGGGAGGGACTGGCCGTACAAAAAGTTCAC20.1295336787564767No Hit
GTTATGCTATATTTACTAAACATATGTTAAACTGAAGGAATCCAGGCATT20.1295336787564767No Hit
AGGTGTCAGGAGTGAGGCCCACTGGGGTCAAGGAACCAAAGGAGGGATAA20.1295336787564767No Hit
ACATAGTATGTACTATGTATTACATAGTAAAAGACCCAAACTCTAAAAGA20.1295336787564767No Hit
GACTTTGCACACAAGACAGAATCGCTTAGTGCCCTGGATTCATTCATTTA20.1295336787564767No Hit
GTAAGGATGACAGGAGAAACATGGGCAAACAGATGCCACAGCCGACCTTC20.1295336787564767No Hit
GTACTGAATCCCATTCTCGCCCCTTCCCGCCATCCAAAAGAAAGGAAAAA20.1295336787564767No Hit
GGGTGAGTCCCCAGGTGGGGATGGGCTGGACCTGTCCGGGTCCCCTGGGG20.1295336787564767No Hit
GGTGTATAGTGTGATGATCTGAAACTCGTGTCCACCGGGAAATGATTACC20.1295336787564767No Hit
GAATGAACGGTCTTTGCACATGGCTCATCTGCTTTCAGCCTGGAATCATT20.1295336787564767No Hit
GCGTAACACTATGCTTAATTTAATTTTGATTGAAGTGCTGCTCTTGCTTT20.1295336787564767No Hit
CACTAGGATCTACCCTCTTAGCAAATTTCAAGAACATAATAACAGTATTA20.1295336787564767No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA20.1295336787564767No Hit
ATTATGGATCACATTTCTTTACTATAATTGTCCTTTTCCTTCTAATGTAA20.1295336787564767No Hit
GGCTTATGGTGGAGTGGAGAGCCAGAGGCTGCTGCCTGTGGTGAAGGCTC20.1295336787564767No Hit
GCTCTACAAGCTTGAGTCAGACAAGCGCCTGGTGGAGGAGAAGGTGAGCC20.1295336787564767No Hit
GCTCATGTCTCTGTAACATCGTTACACGTTACTCTGTTCCATTGTACATG20.1295336787564767No Hit
CCAAAGGAGGGATAAGGGCCAAGGATCTGGGCAGTTGGACCTGGAGCAAT20.1295336787564767No Hit
CCTTCACACTGTGCAGTCTTGCTTCTACTCCCAGTTACCCTTCTATTAAG20.1295336787564767No Hit
CTATAAGGAGGCCCTGGAACGGTGCCGGCAGCTGTGGTGGCGACGAGACC20.1295336787564767No Hit
ACCTATGCCTGCAAGGCCATCAGCGGGAGGAAACGAGGCAGCCGGAATCC20.1295336787564767No Hit
CTCCTAGGAAACAGAGAAGCAAAGCCTGACGGACCGGGAAGGAGACACAA20.1295336787564767No Hit
GCTCGATTCCAGGACCCAAGATCATGACCCGAGCCAAAGGCAGAGGCCCA20.1295336787564767No Hit
GGCTGGGAGAATGCTGGCTGTACGGAAAGGCTGGAGCTGACCACGTGGTG20.1295336787564767No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG20.1295336787564767No Hit
CAATTGTACCCCACATTCTACTCCGTAGAAGCATATCCAGTTTGCAGGAA20.1295336787564767No Hit
AGTCTATAGTGTCTGTATGAGGAGTGGCGGGGTGGGCACTGGGCCTCCCA20.1295336787564767No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC20.1295336787564767No Hit
CTGTTAAGTGTCTACCTTTGGCTCAGGTCATGGTCCCGGGTCATGGGATT20.1295336787564767No Hit
CTTCTGACGCCTTCGAAGATCTCTGCACCGCCCTTAACTCTGTGCCCAGA20.1295336787564767No Hit
CATCAGAACAGATCAGGTTGGATGTGGGAGCATTACACGCGAGGGGCTTT20.1295336787564767No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)