FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_F10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_F10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences870930
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA82050.9420963797320107No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG77060.8848013043528182No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA66370.76205894848036No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA59250.6803072577589473No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT52760.6057892138288955No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG47470.5450495447395313No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG45510.522544865833075No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC43970.5048626181208593No Hit
ATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTC42090.48327649753711555No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT41770.47960226424626545No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC41320.47443537368100763No Hit
GGATGATGGCAATGGTAAGAGGCAATTCTCCAGTATTCAATTACAACAAG38450.4414820938536966No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG37250.4277037190130091No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG36170.4153031816563903No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC34840.40003214954129496No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT34460.39566899750841056No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT33400.3834980997324699No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT32870.3774126508444996No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT32580.37408287692466674No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG32160.3692604457304261No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC32060.36811224782703544No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC30130.3459520282915963No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA28520.32746604204700724No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA28250.3243659077078525No Hit
ATGCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACA24320.2792417301046008No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA24040.27602677597510705No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA23230.26672637295764295No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG23200.2663819135866258No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG22870.26259286050543673No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC22580.25926308658560393No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG22500.2583445282628914No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG22110.25386655643966793No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA22070.2534072772783117No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC21580.24778110755169763No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG21330.24491061279322104No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG20810.23893998369558977No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA20740.23813624516321633No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA20690.23756214621152102No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG20530.235725029566096No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG20300.23308417438829757No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG19440.22320967241913817No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC19150.21987989849930534No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA18860.2165501245794725No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA17920.20575706428760063No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT17810.20449404659387094No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG17710.2033458486904803No Hit
CCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAGGGAAA17320.19886787686725685No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG17250.1980641383348834No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT16960.19473436441505057No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA16660.19128977070487868No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT16500.1894526540594537No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC16500.1894526540594537No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC15580.1788892333482599No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA15550.17854477397724272No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT15450.1773965760738521No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC15330.17601873858978334No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT15210.1746409011057146No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC15120.17360752299266302No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT15050.17280378446028957No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC14730.16912955116943956No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA14730.16912955116943956No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT14720.1690147313791005No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC14620.16786653347570987No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC14540.16694797515299736No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC14390.16522567829791143No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC14340.1646515793462161No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT14260.16373302102350362No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC14260.16373302102350362No Hit
ATACAGTTCTTGGAAAGGATGCAGGTGCATTGACTGAAGATCCAGATGAA14180.1628144627007911No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA13670.1569586533934989No Hit
CTACCTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCC13180.15133248366688482No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA13080.15018428576349419No Hit
GATCTTATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAA13060.14995464618281606No Hit
GGATGATACAGTTCTTGGAAAGGATGCAGGTGCATTGACTGAAGATCCAG12980.14903608786010358No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA12980.14903608786010358No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC12810.1470841514243395No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC12710.14593595352094887No Hit
CCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTT12430.1427209993914551No Hit
TCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAG12370.14203208064942074No Hit
CCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCA12160.1396208650523004No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG12110.1390467661006051No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA12070.13858748693924883No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA12010.13789856819721447No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT11880.13640591092280666No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA11870.1362910911324676No Hit
CTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCC11450.13146865993822696No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA11240.12905744434110664No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA11130.12779442664737695No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA11040.12676104853432538No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG11040.12676104853432538No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC10900.1251535714695785No Hit
GAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACACCAATAC10670.12251271629178005No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATA10340.11872366321059098No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG10140.11642726740380974No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG9960.1143605111777066No Hit
GTATCATCCACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGAT9790.1124085747419425No Hit
TACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTT9780.11229375495160346No Hit
ATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG9690.11126037683855189No Hit
CTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGAGAATAGAACGAT9580.1099973591448222No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT9570.10988253935448314No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG9470.10873434145109251No Hit
TCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAA9440.10838988208007531No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA9430.10827506228973625No Hit
GTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCC9370.10758614354770188No Hit
ATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATGCA9370.10758614354770188No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA9330.10712686438634562No Hit
TCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATAT9280.10655276543465031No Hit
GCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACAC9220.10586384669261593No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA9030.10368227067617375No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG8970.10299335193413937No Hit
GAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCA8930.10253407277278312No Hit
GCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACT8840.10150069465973155No Hit
GAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATG8780.10081177591769717No Hit
GTTCATTGATGCTTAATGCATATCTCTTGTCTTCTTTGCCCAAAATGAGA8770.10069695612735811No Hit
GAATGAAGTGGATGATACAGTTCTTGGAAAGGATGCAGGTGCATTGACTG8770.10069695612735811No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACAGATGTACCAG8750.10046731654668No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT8710.10000803738532374No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGC100.0070756213145.10331
AGTAGAA11050.0110.9613651
GCAGGTA5750.0107.170132
CAGGTAC6050.0101.7564853
GGTACTG6200.099.283225
CCGCACG554.1472958E-1092.30661145
AGCGAAA1650.087.941411
TAAAGTC259.4241457E-486.9123
AGCAGGC2000.086.9123
GGTCAAA9150.083.2561
ACTGATC7550.081.525928
GCAGGCA2150.080.843734
GTACTGA7800.078.9174356
AAATATA10200.075.2582865
CGAAAGC2350.073.967663
GCGAAAG2100.072.497442
AGGTACT9500.067.848464
TACTGAT9450.066.667037
GTCAAAT11550.066.534452
GTAGAAA18700.065.131392
Produced by FastQC (version 0.11.5)