FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_F09-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_F09-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences822598
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG11660.14174602904456368No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA8920.10843692787971768No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA8480.10308802112331905No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC8400.10211549262215565No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGCG657.32813E-444.62488145
CAATTCG4150.043.625579
GGATAGC4200.037.933475
TATATTC4900.032.5282443
GTACTGA4550.031.8322836
GTACGAG950.004714863430.542111
AGCGAAA2202.2519089E-829.6744371
TAGTACG2701.72804E-1029.512784
GTAGAAA7900.029.3822841
AGTAGAA6200.029.2489971
GATATAC1000.00605850229.0150051
GTTGCGG1000.006107327528.9673798
GATAGCA5600.028.4501046
GTGATCG1804.7399553E-628.1627279
GACAGTA4050.026.8216467
TTAGACA6000.026.5615024
CGAAAGC3607.2759576E-1226.178162
CAATCGA1958.828809E-625.9963679
TAGACAG5850.025.9963655
CCTAATA1409.998408E-425.8904882
Produced by FastQC (version 0.11.5)