FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_F05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_F05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATTTATAGCAAAGATTCGCCTTGCCACAACTACTTAATTGCCTGTGTT14.3478260869565215No Hit
CCCTTTGTCAGTCATAAAGAGATTGATTCTGTAAACAATGCTGTGCAAAT14.3478260869565215No Hit
GCCTTCTTCAATGTCTTCAGAAGATTGAGAGCATGATTGAGGCCGAGTCT14.3478260869565215No Hit
TCCTCACGCATTCCCCTTGGTCTTGTGTTGAGAATCGCACGATTCCTCTT14.3478260869565215No Hit
ACCAGGTACTGATCCAAAATGGAAGACTTTGTGCTAAAAAGCTTCAATCC14.3478260869565215No Hit
TATATCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTA14.3478260869565215No Hit
AATTCCACTCTTTAAATGAACTGACTCGTCTATTGACCCACACGCGCACT14.3478260869565215No Hit
TAAGTATGACCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGC14.3478260869565215No Hit
GTCAGTAAGTATCCTAGAGTCCCGTTTTGGTTTCATTACACCCACAACGT14.3478260869565215No Hit
GTAGAAACAAGGTAGTGTTTAAACAATTCGACTCTAATTGATGTAGATCC14.3478260869565215No Hit
GATTGGAGGAGTAAGGATGGTGGACATCCTTAGACAGAATCCAACTGAGG14.3478260869565215No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA14.3478260869565215No Hit
GACTAGAATCATCCGCGGTTAATGCTGACATAGTTTCGAACGAACAACAG14.3478260869565215No Hit
ACTCAGGACAATATTCCAAAAAAAAACTATTAATGAAAAACAAAGCGATT14.3478260869565215No Hit
CTGAGGAGCAGCGTGGATTAAACAACAAAGAGCCATAAGTCCGCAAGACA14.3478260869565215No Hit
CAAAGAAACTACGATATGGTCTCCCTGCAATGCCAAAATAAAACAACTTC14.3478260869565215No Hit
GTATTGGAGTAACAGTGATAAAGAACAACATGAAAAACAATGACCTTGGA14.3478260869565215No Hit
AGAAACAGACAGCCTGTCTGCCACCATAAGAATTCCGATGGGCATCAAAA14.3478260869565215No Hit
ATGAAGTGGGGGCAAGAATACTGACATCAGAGTCACAGCTGGCAATAACA14.3478260869565215No Hit
TTTGTAAACAGTGTGGTGAGGGAAGGCCTCTGTGAGAAGGTGAAGGCAAT14.3478260869565215No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG14.3478260869565215No Hit
GTATGCTAGACTCCCGATATCCGTACAATTCCAAAAATACCTCCAGTTCC14.3478260869565215No Hit
ACACGCTCGAGTTACCCCCTACGGACGCTGTCTCTGACTGCGTCAGCGGT14.3478260869565215No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)