FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_F05-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_F05-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502287
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1900.053.4905321
CGAAAGC2950.034.4103283
GCGAAAG3050.033.3053552
ATATACC1551.469889E-632.7224854
TAACGGA900.003637093132.203084
TAGCCTC1652.3962366E-630.7362425
AATATAC2252.8083377E-829.0029893
TATATAC5050.027.280043
GTCTAAC1100.009606020526.3979241
GTGCGAG1100.00970925926.3401059
GTATATA4150.026.2389011
TATATGA5750.025.2024124
ACTAGGC1450.001231274624.9801718
CAACGTA1450.001231989624.9776849
ATATGAG5900.024.5592235
TTAGGAC2701.6438207E-724.1691593
TATACAG3301.7807906E-924.1499045
GTCCAAT1550.001793621623.4175151
CATATAT4353.6379788E-1223.363681
CCTATGC1902.1791979E-422.9130822
Produced by FastQC (version 0.11.5)