FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_F04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_F04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATGATCGTAATAATCCCCGACATGACCCCAAGCACGCAGCTGTCGCT11.2048192771084338No Hit
TATATGGACTTAGCGTGAGCGCATGGAAGACAAAAGAGATGATCTCAGCC11.2048192771084338No Hit
ATTTTAGGATTTGTGTTCACGCTCACCGTGCCCAGTGAGCGAGGACTGCA11.2048192771084338No Hit
GTGTCCTTACGAATCCACTGTACCGGCTTCTGGTTGCCTTAGGGACAAGA11.2048192771084338No Hit
CGCCCAAGACCGTAATTAGCCTGTTTCACCTGCCCAGCATCAGCCCCGGA11.2048192771084338No Hit
TCAGGGAGCAGGTAGGGCGGAGGGGGGGAGAGACTGGGCGGCAGAGGGCT11.2048192771084338No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGCCCAT11.2048192771084338No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTGTGATTGGG11.2048192771084338No Hit
GCACTCCCGCTGGTGAGTGGGGGGTGCATGGCGTCGGGTCAGGGCATCCA11.2048192771084338No Hit
GATTACACTGAAGTTCGCTGTGAGTACTATGTTCTTTTCCATGTTCGCCT11.2048192771084338No Hit
ATATAAGAGAGAAATACTTTTCTTTTCTTTTTTTTTTTTTTAATGATTTT11.2048192771084338No Hit
CCTGCAGATGGCCCCAAATGACCTACTGGCACCTGCCCTGCTCCATACAC11.2048192771084338No Hit
TCGCCGGACAGAAGCCTCCACCCCAGCTTTGACGTCATCTGGATCTTCAG11.2048192771084338No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTAATTATTTT11.2048192771084338No Hit
CATTGGGATCAGCTGAAAGTCGTCCATGGCTGCACAGGAGGCAGTGCGCA11.2048192771084338No Hit
GTGGGGGGGGGTGGTTATGTGGGGTCGGTTCAGTTTGTATGCGGGCGGGG11.2048192771084338No Hit
GCGTAGATGACATCCCATTAACAGGAAGGCATTACTGCCTCGTCCCCGAA11.2048192771084338No Hit
CAGTACCCTGGAACTGAGAAGCTGATACTGGGCCAGAAGGGGCAGGGGTG11.2048192771084338No Hit
GGTCTCTCGTAGTGGCGAGCCATGACTGTCAGGAGGCCCAGGGAACGTCT11.2048192771084338No Hit
AACTTATACAATATACGGAATGTTCACATGCCTGAGGTCTGGTTAATATG11.2048192771084338No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG11.2048192771084338No Hit
CGATTATCCAGCAGCAAGTGTTCCCGCCTTCAGCTGCCAGCCGCCTCCTC11.2048192771084338No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCAGTACCACCACGACGGCCCCTTGC11.2048192771084338No Hit
AGAAAATGTTGACACCTGCTGTACCACAGGTGAGCCTTGAGGGTATTCCG11.2048192771084338No Hit
CTCATAACCCCACAGCACTGGAGGCCAGACGTCGACACTGAGTCCTGCTG11.2048192771084338No Hit
CAGGAGTTCTGTTTTCGCCCTGTTGACTTTGAGATGGCTGTTAGAATTCT11.2048192771084338No Hit
CTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGTACTATGTTCTTT11.2048192771084338No Hit
TTCATGCCTTGAAACAATGAATCCCTTTGTCAGTCATAAAGAGATTGCTT11.2048192771084338No Hit
GCTTATCTATATCCTCTGTCCAGGGTTGGATTTACGAACAGCCCTCTCTT11.2048192771084338No Hit
ATTAACTAAATAGCTGGGTAGATTGATGTAGTGTGGGTATAAAGACTGAT11.2048192771084338No Hit
ACATTAAATTAAAATTATTACTTCCGACACAGAAGCATGGAAGTGAGGAG11.2048192771084338No Hit
GTTCTGGTGGGGCAGCAGCAAAGGGGAGAAGGTTTATTATTTGGCCAGTG11.2048192771084338No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.2048192771084338No Hit
ATTTTTAATTTACAATCATAACTCTGGCTACCTTGACTTAGATTAAATAC11.2048192771084338No Hit
CTCTAAGGACAGCTTGGGCATGTCTGTCACCTTCCAGACCTGTGTTTCAC11.2048192771084338No Hit
GGAACAATAGCAATGGAGTTAATCAGAATGATCAAACGTGGAATCAATGA11.2048192771084338No Hit
GTTTTCATGTCTTCTTGTGTAGGGGTCATGATAATCTTATCTGTATCATT11.2048192771084338No Hit
GTCTGTTGTTTTTTAAAAAACCAGTTTAGGATAATCCTTATGCCAAAGAA11.2048192771084338No Hit
CAGGAGATCTCCTAAGGGGAAAATGCAGAATGGCAAGAGAGGACAGACGG11.2048192771084338No Hit
AAATAAAGCACAACAATAAAAATTCAAACATTATCAAATTCTAAAAAACG11.2048192771084338No Hit
CTTAGAAGGGAGGCAAGAAAACATTACACCAACATCAACAACTAACACTT11.2048192771084338No Hit
AAAGAGACTTGGTCCGTACAACAAGGGTTCTCCCAGTAGCCGGCGGCACA11.2048192771084338No Hit
ACACTTCTGCTGTAAAATAGTTCCTCCTCATGCTTGCGATATGTTCAATC11.2048192771084338No Hit
GGTGTAAAGGATGGGGCAGTGCGGTGGGCGACTTTGGTCGGCAAGNTNGN11.2048192771084338No Hit
TCCTAAAAATTACAGCGCAAAAAGCCATAAGAACCAAATTAACTTATACT11.2048192771084338No Hit
TCCATCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCAT11.2048192771084338No Hit
ACACTTATCAATGGATAATCAGGAACTGGGAAATTGTGAAAATTCAATGG11.2048192771084338No Hit
GATTATGGATCACATTTCTTTACTATAATTGTCCTTTTCCTTCTAATGTA11.2048192771084338No Hit
GGAACAGGTGCAGGAGCAGGTGCTGGAGCAGGAGCAGGTGCAGGAGCAGG11.2048192771084338No Hit
GTCCTCCACTCCGGTGCCGTCTGGAAAAGGATAGGCGTCTCAGTTCCAGT11.2048192771084338No Hit
CAGTGGGGCGCGTCGGTCGAGGGGGCTCGCGGCGCGACTCCCCCGGGGGG11.2048192771084338No Hit
GGTGAGGTGGGCTTGGCGGTCAGATTTATGGAGGCCGGGGTGTGTGCCTC11.2048192771084338No Hit
GACGAGAAGAATGGCCATGAGTCCAGCGGCAGCAAGGCCCCCAATCGCCG11.2048192771084338No Hit
GTTTTAGGATGGGGAGACCCAGCCTCTGGGTGTCAGACTGAGGGAGGGAG11.2048192771084338No Hit
CTTGCAAGTGGGAGTTCAGAGAGGAGAACAGTTGCTGAGAACAGTAGCTG11.2048192771084338No Hit
CCTGCGAGATGTTCAGATTTGCACTGGGAAGGAGCGTGAGGTGCGTCGAC11.2048192771084338No Hit
TGCTGGGATAGATGGCAGTGAGCCGACGGACTGGCAACAAGGCTCTCCAG11.2048192771084338No Hit
ACTCATGTCAGCTGATTCATTTACTCAAGACTCTCCAAAGCTGGGTAGCT11.2048192771084338No Hit
TACATACACCGCACAGACAAGATCCTGAGGAGGTAGAGTCTGAGGACAGT11.2048192771084338No Hit
GGAAATAATTGTGGCCATGCTATTCTGACAGGAGGATTGGATGATCAAGG11.2048192771084338No Hit
ACACGGAGCCGCCGCCTGGACAGCCCCCAGAGCACTGGCTCCCCCGAGTC11.2048192771084338No Hit
GTTCAGTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA11.2048192771084338No Hit
AAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTCATATA11.2048192771084338No Hit
GGTATTCTCACAGGAGGATTGCATGATCAAGGCAGTTAGGGGCGATCTGA11.2048192771084338No Hit
CCTCCAGATTTGCTTTCCTTTTTTAAGGTTGTTTGGCTATTCAGGCTCTT11.2048192771084338No Hit
ATTTTATACTTGATTTATGAACCCATTCAGCTGAATTTCCAGGGCATCTC11.2048192771084338No Hit
ATCTCAAGCTTAGTATCAACTGGATCAATCTCCTGGTTTACTTTCTGACA11.2048192771084338No Hit
GGCCATTCCTATTCCACCATGTGACGGCCAGAGGTGATACCCTCACTCGG11.2048192771084338No Hit
CCTTGTTGGCCTAAATAGAAGTGAGAAAAGAAAAAGGAAGTAAAATACAA11.2048192771084338No Hit
CCTTTGTACCTGCTATGCTCTCTGCTTATTTTGTACTCTCTCAGATATTC11.2048192771084338No Hit
CAAGAATACTGCCATCAGAGTCACAGCTGGCAATAACAAAAGATAAGAAA11.2048192771084338No Hit
TAATAGGCCCTCTGTGCGTTCTATTGGACCTGTCGATCATGGAAAAGACC11.2048192771084338No Hit
GAGGGGATGAGTAAGGATTGTTGAAATAATGAGAAATACTCCAACTTATG11.2048192771084338No Hit
CTACAGCAATGGTATAATTATCTTATTATAAAAAGTTTGGGCCTCCAAGA11.2048192771084338No Hit
GACCAGGAACGTGCCCTCTACCTACTCTTTCGGAGGACGGTGCACTTGGG11.2048192771084338No Hit
GCATTTGCGGTGCTGCCTTCCTCTCCTTTATTGCCCGGGCTCAGGTCTGG11.2048192771084338No Hit
ATCCTTGACAATCCCATATGGGAATGTGGGCGGGTCTCATGACTTTTCCC11.2048192771084338No Hit
GGGGGATTCTGCCGTTCGGGCTCCCTGTGGCTTAATGGTTGTTTGCTGAG11.2048192771084338No Hit
GGCGTGGTGGGTGTGGGGCCTGGGGTGTGGGTGCGGCGGAGGGGCGGTGT11.2048192771084338No Hit
CTTTACTTGATTTTGCTGTCTCGTTAACTTGTATAGCCAAGAGCTCCTGT11.2048192771084338No Hit
AACGCAGGGATTCAATGTTTTGAGTTTAGCAAAATTGAATAAAAACAGAA11.2048192771084338No Hit
CTCTCATCCACTTTCCGTCTACTCTTCTATATATGGGTCCTCCTGTTTTC11.2048192771084338No Hit
AAGGGGGCAGTGTGTGTAAACCCCTTTCCACTCGGTGCCCATTGCAGACT11.2048192771084338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGCC50.0145.03
CATTTTC50.0145.0145
GGCCCTC50.0145.06
CCTCTGT50.0145.09
AATAGGC50.0145.02
AGGCCCT50.0145.05
TAATAGG50.0145.01
CCCTCTG50.0145.08
TAGGCCC50.0145.04
GCCCTCT50.0145.07
Produced by FastQC (version 0.11.5)