FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_F03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_F03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATTTACTAAACATATGTTTAACTGAAGGAATCCAGGCATTCAAGGT15.555555555555555No Hit
AATATATTCAATATGGAGCGCATAAAAGAACTGCGAGATCTAATGTCGCA15.555555555555555No Hit
GGATACACCAGGGACACAGTAAACAGAACCCACCAATACTCAGAAAAGGG15.555555555555555No Hit
TATATGGACTGCTAGGGAAAAATTTCTCGAATAGATTGCCGCACTTCTGG15.555555555555555No Hit
CACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCAT15.555555555555555No Hit
TTGTGTGAGACGCTTATGGGTGCCCTGGTCAGGTTGTTTCTGAACCACAC15.555555555555555No Hit
GGGAGACACACAAATTCAGACGAGAAGATCATTTGAGTTTATGCAGCTGT15.555555555555555No Hit
CTACCCTCATGGACTCAGGCCCTCCTTCCGTAGAAGGCCGTCTGTTCAAA15.555555555555555No Hit
GTCTCTGGGGCAGGGGAGGGTGTGTGTGTGTGTGTGACAGGTGGGCTTCA15.555555555555555No Hit
TTGGGAAAGTGTGCAGGACCTTACTGGCAAAATCTGTATTCAACAGTCTA15.555555555555555No Hit
ATGAGAAGCAATGTTCTAGATTCAGCCGAAAACCCCTCAAGTTGTGGAGA15.555555555555555No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT15.555555555555555No Hit
GAGCTGTCACATATCGCTATCCTTTGGGCATTTTGCTTCTCACTCTACCC15.555555555555555No Hit
ACCCTGAAGTTCGCTTTCAGTACTATGTTCTTTTCCATGATCGCCTGGTC15.555555555555555No Hit
GTTATAAATGCAAATAAAATAACTAAGAACAAAAAAGAATAAAATGATAA15.555555555555555No Hit
GTACTTGTTGTAGCGCCATTCCTATTCCACCATGTTACGCCCAGAGGTGA15.555555555555555No Hit
GGTCCTCGTGCTCAGGCAGGGTGAGCTGCGGGAGCACTCAGAGCTCATGG15.555555555555555No Hit
CCTATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT15.555555555555555No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)